| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,562,043 | 0 | A | C | 57.1% | 9.0 / 15.6 | 14 | G276G (GGT→GGG) | eutG | ethanolamine utilization ethanol dehydrogenase EutG |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/8); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTTGGCAGCAACATGGCGTTCGCGAGACCGTGCGGAATATGCAGCGCCGCGCCCGGCTGATGCGCCATCGCGTGGCACAACCCAAGACCCGCACTGGAAAACGCCATTCCCGCCATACATGAAGCCAGCAACATGCTCTCGCGCGCGGCAAGGTCGTG > NZ_CP009273/2561956‑2562114 | cGTTGGCAGCAACATGGCGTTCGCGAGACCGTGCGGAATATGCAGCGCCGCGCCCGGCTGATGCGCCATCGCGTGGCACAACCCAAGAcc < 1:645278/90‑1 (MQ=255) caACATGGCGTTCGCGAGACCGTGCGGAATATGCAGCGCCGCGCCCGGCTGATGCGCCATCGCGTGGCACAACCCAAGACCCGCACTGGa > 2:128927/1‑90 (MQ=255) gCGTTCGCGAGACCGTGCGGAATATGCAGCGCCGCGCCCGGCTGATGCGCCATCGCGTGGCACAACCCAAGACCCGCACTGGAAAACGcc > 2:612045/1‑90 (MQ=255) gTGCGGAATATGCAGCGCCGCGCCCGGCTGATGCGCCATCGCGTGGCACAACCCAAGACCCGCACTGGAAAACGCCATTCCCGCCATACa > 2:609780/1‑90 (MQ=255) gCGGAATATGCAGCCCCGCGCCCGGCTGATGCCCCATCGCGTGGCACAACCCAAGCCCCGCACTGAAAACCGCCATTCCCGCCATACATg < 1:90317/90‑1 (MQ=255) tGCAGCGCCGCGCCCGGCTGATGCGCCATCGCGTGGCACAACCCAAGACCCGCACTGGAAAACGCCATTCCCGCCATACATGAAGCCAGc > 2:424647/1‑90 (MQ=255) tGCAGCGCCGCCCCCGGCTGATGCGCCATCGCGTGGCACACCCCAAGCCCCGCACTGGAAACCGCCATTCCCGCCATACATGAAGCCAGc < 2:596805/90‑1 (MQ=255) cAGCGCCGCCCCCGGCTGATGCCCCATCGCGTGGCACACCCCAAGCCCCGCACTGGAAAACGCCATTCCCGCCATACATGAAGCCAGCaa < 1:300295/90‑1 (MQ=255) gccccgcgccGGGCTGATGCCCCACCGCGTGGCACACCCCAAGCCCCGCACTGAAAACCCCCATTCCCGCCATACATGAAGCCAGCAACa < 1:251895/87‑1 (MQ=255) cgccgcgccCGGCTGATGCGCCATCGCGTGGCACAACCCAAGACCCGCACTGGAAAACGCCATTCCCGCCATACATGAAGCCAGCAACAt > 2:263181/1‑90 (MQ=255) gcgcCATCGCGTGGCACAACCCAAGCCCCGCACTGGAAAACGCCATTCCCGCCATACATGAAGCCAGCAACATGCTCTCGCGCGCGGCaa < 2:164687/90‑1 (MQ=255) gccccATCGCGTGGCACAACCCAAGCCCCGCACTGAAAAACGCCATCCCCGCCATACATGAAGCCAGCAACATGCTCTCGCGCGCGGCaa < 2:535895/87‑1 (MQ=255) tCGCGTGGCACACCCCAAGCCCCGCACTGAAAACCGCCATTCCCGCCATACATGAAGCCAGCAACATGCTCTCGCGCGCGGCAAGGTCGt < 2:426459/90‑1 (MQ=255) cgcgTGGCACACCCCAAGCCCCCCACTGAAAAACGCCATTCCCGCCATACATGAAGCCAGCAACATGCTCTCGCGCGCGGCAAGGTCGTg < 2:614002/90‑1 (MQ=255) | CGTTGGCAGCAACATGGCGTTCGCGAGACCGTGCGGAATATGCAGCGCCGCGCCCGGCTGATGCGCCATCGCGTGGCACAACCCAAGACCCGCACTGGAAAACGCCATTCCCGCCATACATGAAGCCAGCAACATGCTCTCGCGCGCGGCAAGGTCGTG > NZ_CP009273/2561956‑2562114 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |