| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,118 | 0 | C | A | 66.7% | ‑3.9 / 18.0 | 18 | A422A (GCC→GCA) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (5/1); new base A (0/12); total (5/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTA > NZ_CP009273/3214038‑3214191 | gttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATc < 2:57182/90‑1 (MQ=255) tttGCCAGCGAGTTGTCCCGCCGGCGCGTACGGGGGGCCGAAACGCTCAAAAACGCAAAACCGACCCGAATTAAACCGCcactgacactg < 2:717069/90‑1 (MQ=255) tttGCCAGCGAGATTTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTAAATAACGCAAAAACGATCCGCATTGAACCGCcactgacactg < 2:558778/90‑1 (MQ=255) ttggCCAGGGAGTTTTCCCCCCGGCGCGTACGGGGGGCCGAACCGCTAAAAAACGCAAAAACGCCCCGCATTAAACCGCcactgacactg < 2:544716/87‑1 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 2:309041/1‑90 (MQ=255) gagtTGTCCCGCCGGCGCGTCCGGGGGGCCGGAACGCTCAAAAACCCAAAAACGACCCGAATTGAACCGCCACTGACACTGACCATTGaa < 2:631580/90‑1 (MQ=255) agttGTCCCGCCAGCGCGTCCGGGTGGCCGGAACGCTAAAAAACCCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 2:691795/87‑1 (MQ=255) agttGTCCCGCCAGCGCGTACTGGGGGCCGGAACGCTCAATAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 2:7768/87‑1 (MQ=255) agttGTCCCCCCGGCGGGTGCTGGGGGCCGGAACGCTAAAAACCCCAAAAACGACCCGAATGAAACCGCCACTGACACTGACCATTGAAc < 2:376180/87‑1 (MQ=255) agttGTCCCCCCGGCGCGTCGGGGGGGCCGGACCCCTAAAAACCCCAAAAACGACCCGCTTTAAACCGCCACTGACACTGACCATTGAAc < 1:65737/87‑1 (MQ=255) ctGTTCCGCCGGTGCTTCCTGGCGGCCGGACCGTTAAATACCTAAAAACCCCTCCGTGTTGACCCGCCACTGACACTGACCATTGAACAg < 2:611214/89‑1 (MQ=255) gCCCGCGCGTACGGGGGGCCGAACCGCCAAAAAACCAAAAAACGTCCCGCATTAAACCGCCACTGACACTGACCATTGAACAGTGTGAAc < 1:144675/90‑1 (MQ=255) gcgcGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGt > 1:190161/1‑90 (MQ=255) cgcgTACGGGTGGCCGGAACGCTAAAAAACCCAAAACCGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg < 2:190161/90‑1 (MQ=255) gtggCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcg > 2:261362/1‑90 (MQ=255) ggCGGGAACGCTAAAAAACCCAAAAACGACCCGAATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcggc < 2:296522/90‑1 (MQ=255) gAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTa > 2:122510/1‑90 (MQ=255) gAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTa > 1:158212/1‑90 (MQ=255) | GTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTA > NZ_CP009273/3214038‑3214191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |