| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,703,332 | 0 | A | C | 58.3% | 4.0 / 12.2 | 12 | G168G (GGT→GGG) | eptB | kdo(2)‑lipid A phosphoethanolamine 7''‑transferase |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/7); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCC‑AAA‑CC‑ATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACA > NZ_CP009273/3703243‑3703409 | ccGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCC‑AAA‑CC‑ataata > 2:81375/1‑90 (MQ=255) ggCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCC‑AAA‑CC‑ATAATACCCGCCAGTacg > 1:491472/1‑90 (MQ=255) ccGCCGGCCCCCCCCCCCCTTTTCTTCTGGGGAATACCCAACAAACAAACCGTTCCAA‑AAC‑CC‑AAAACCCCCGCCAGTACGACGACCGcc < 1:45251/89‑1 (MQ=255) ttttctGCGGAATACCCAGAAAACAAATGGGT‑CCC‑AAA‑CCAAAAATCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTg < 2:228976/88‑1 (MQ=255) ttctGCTGGATATCCAGCAAACGAATCGGTGCCC‑AAA‑CC‑ATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTggg > 1:95250/1‑90 (MQ=255) ttctGCTGGATATCCAGCAAACGAATCGGTGCCC‑AAA‑CC‑ATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTggg > 1:95546/1‑90 (MQ=255) ggATATCCAGCAAACGAATCGGTGCCC‑AAA‑CC‑ATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCa > 2:179829/1‑90 (MQ=255) aaaaCAAAGCGGTCCCA‑AAC‑CC‑AAAACCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAg < 1:290350/89‑1 (MQ=255) aaCGAAGCGG‑GCCC‑AAA‑CCAAAAATCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCa < 1:256832/90‑1 (MQ=255) aaCAAATCGG‑GCCC‑AAACCC‑AAAATCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCa < 2:196662/90‑1 (MQ=255) cGAATCGGTGCCC‑AAA‑CC‑ATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAg > 2:130322/1‑90 (MQ=255) caaTCGGGCCCCAAAA‑CC‑AAAACCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAg < 2:95546/89‑1 (MQ=255) c‑AAA‑CCAATAATCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACa < 1:167879/90‑1 (MQ=255) | CCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCC‑AAA‑CC‑ATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACA > NZ_CP009273/3703243‑3703409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |