| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,594,161 | 0 | C | T | 56.8% | 1.2 / 37.3 | 37 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional activator BglJ |
| Reads supporting (aligned to +/- strand): ref base C (3/13); new base T (21/0); total (24/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.57e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-07 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCG > NZ_CP009273/4594078‑4594239 | cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc < 2:269974/90‑1 (MQ=255) cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg < 2:130650/90‑1 (MQ=255) tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGcc > 1:35818/1‑90 (MQ=255) tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGcc < 2:629281/90‑1 (MQ=255) tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGaa < 2:35818/90‑1 (MQ=255) ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAg > 1:227550/1‑90 (MQ=255) tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAgcgc > 2:687789/1‑90 (MQ=255) tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc < 1:166318/90‑1 (MQ=255) tataCCAGTCAGGAGCCATTTCAGGATGCGATGTCGCGGATCTCGTTTGCGGCGGGGATTATCTTTTTTTGTTCCCTGAggagggagggg > 1:3177/1‑87 (MQ=255) ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag < 1:359676/90‑1 (MQ=255) ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag < 1:501292/90‑1 (MQ=255) cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga < 1:606539/90‑1 (MQ=255) aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCagagag > 1:193346/1‑90 (MQ=255) aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTTCCTTAGAAGTGAGGGCagagag > 1:550714/1‑90 (MQ=255) aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTTAGAAGAGAGCGCcgagag > 1:604275/1‑90 (MQ=255) aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCATGGGAAGGGGGGGCagagag > 2:649398/1‑90 (MQ=255) gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCCGGGAgg > 2:501292/1‑90 (MQ=255) gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAgggg > 2:268167/1‑90 (MQ=255) gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAgg > 2:543542/1‑90 (MQ=255) gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCAGAGAgg > 1:189178/1‑90 (MQ=255) tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGGGGAGTGGGCGCAGAGgggg > 2:329115/1‑90 (MQ=255) tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCCTTTTTTTTTTTTTTTCCCTGGGAAGGGAGGGGagggaggg > 1:321120/1‑90 (MQ=255) ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGGtt > 2:359676/1‑90 (MQ=255) gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATc < 2:90240/90‑1 (MQ=255) cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCAGAGAGGGGTTAtttt > 1:524881/1‑90 (MQ=255) aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGCTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg > 1:101255/1‑90 (MQ=255) tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc < 1:50641/90‑1 (MQ=255) tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc < 1:50640/90‑1 (MQ=255) gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCctgact > 2:165248/1‑90 (MQ=255) tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCctgactga > 2:619734/1‑90 (MQ=255) gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTGAGAAGGGAGCGCAGAGAGGGGTTATCTTTCCTGACTGaa > 2:668989/1‑90 (MQ=255) ccATGTCGCGGATCTCGTTTGCGGCGGACCTTTTTTCTTTTTCTGCCCTGAGAAGGGAGGGTCGGGAGGGGTTATCTTGGCCGACTGAAc > 2:409692/1‑90 (MQ=255) gcgGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGAt > 2:327892/1‑90 (MQ=255) ctcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGtt < 1:708889/90‑1 (MQ=255) cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc < 2:550714/90‑1 (MQ=255) cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc < 2:74673/90‑1 (MQ=255) ggTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCg > 2:599473/1‑90 (MQ=255) ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg > 2:190207/1‑90 (MQ=255) | CCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCG > NZ_CP009273/4594078‑4594239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |