Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	221,335	T→C	noncoding (1078/1542 nt)	BW25113_RS01010 →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	221,335	0	T	C	100.0%	57.4 / NA	21	noncoding (1078/1542 nt)	BW25113_RS01010	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/13);  total (8/13)

TGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCC  >  NZ_CP009273/221249‑221419                                                                                       |                                                                                     tGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGaa                                                                                   <  1:277764/90‑1 (MQ=255)  gACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGaaa                                                                                  <  2:621864/90‑1 (MQ=255)  gACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGaaa                                                                                  <  1:440121/90‑1 (MQ=255)       ccACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTg                                                                             <  2:690044/90‑1 (MQ=255)       ccACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTg                                                                             <  2:351906/90‑1 (MQ=255)            gAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTa                                                                        <  1:115055/90‑1 (MQ=255)            gAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTa                                                                        <  2:339228/90‑1 (MQ=255)            gAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTa                                                                        <  1:716053/90‑1 (MQ=255)                        gaTGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACg                                                            <  1:503494/90‑1 (MQ=255)                           ggATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAgc                                                         >  2:31400/1‑90 (MQ=255)                              ttGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCa                                                      <  1:109339/90‑1 (MQ=255)                                ggTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAAc                                                    >  2:197758/1‑90 (MQ=37)                                ggTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCGGTCGTCAGCTCGTGTGGCGAAATGTTGGGTTAAGTCCCGGAAGGAGCGCAAc                                                    >  1:688790/1‑90 (MQ=11)                                                gtgAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGc                                    >  1:103745/1‑90 (MQ=18)                                                        ggTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTc                            >  2:182513/1‑90 (MQ=18)                                                        ggTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTc                            >  1:15407/1‑90 (MQ=18)                                                                aTGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCggg                    <  2:318254/90‑1 (MQ=18)                                                                aTGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCggg                    >  2:518685/1‑90 (MQ=18)                                                                      gtcgtcAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCa              >  1:593337/1‑90 (MQ=18)                                                                        cgtcAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCaaa            <  1:41498/90‑1 (MQ=18)                                                                            aGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGgag        <  1:603700/90‑1 (MQ=18)                                                                                  tgttgcGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGcc  <  1:370951/90‑1 (MQ=18)                                                                                  tgttgcGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGcc  <  1:31400/90‑1 (MQ=18)                                                                                  tgttgcGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGcc  <  2:51421/90‑1 (MQ=18)                                                                                       |                                                                                     TGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCC  >  NZ_CP009273/221249‑221419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑