| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,819,318 | G→A | intergenic (‑124/+79) | ves ← / ← spy | environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,819,318 | 0 | G | A | 80.0% | 14.5 / 3.7 | 20 | intergenic (‑124/+79) | ves/spy | environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy |
| Reads supporting (aligned to +/- strand): ref base G (4/0); new base A (0/16); total (4/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.06e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-03 | |||||||||||
TCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTAT > NZ_CP009273/1819230‑1819400 | tCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGagaaaaga > 1:171346/1‑90 (MQ=255) aTCGGCACCTGGGGTGTTTTACTTTAGTGGTGATCGGTTTTTTTTTACAACTTTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCAc < 1:212392/90‑1 (MQ=255) ccGGCAACTGGGGGATTTTAAGTTAGGGGTGATCGGGTTTTTTTTAAACCTGTTG‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCAcc < 1:687086/89‑1 (MQ=255) aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTa > 2:106117/1‑90 (MQ=255) gttaTTTACGTTAGTGTTGACCAGTTTTTTTTTACAACTTTTT‑GAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 2:119699/87‑1 (MQ=255) gtgtTTTACTTTAGTGGTGATCAGGTTTTTTTTACAACTTTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 2:638712/90‑1 (MQ=255) gtgtTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 2:313130/90‑1 (MQ=255) gtattttACGTTGGTGGTGATCGGGTTTTTTTTACAACTGTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 2:601411/86‑1 (MQ=255) gTGATTTCCGTAAGGGGTGACCAGCTTTTTTTTACAACTTTTG‑GAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCaa < 1:391707/90‑1 (MQ=255) gTGATTTACTTTAGTGGTGATCAGGTTTTTTTTACAACTGTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 2:37933/90‑1 (MQ=255) gTGATTTACTTTAGGGGTGATCGGTTTTTTTTTACAATTTTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 1:66680/90‑1 (MQ=255) gTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTT‑GAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 2:130156/90‑1 (MQ=255) tGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAg < 2:272135/90‑1 (MQ=255) ttATGTTGGTGGTGATCGGTTTTTTTTTAAATTTGTTG‑GAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg < 1:606297/90‑1 (MQ=255) ttACTTTGGGGGTGA‑CAGGTTTTTTTTAAAAATTTTTGGAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg < 1:516571/90‑1 (MQ=255) ttACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGGAg > 2:604359/1‑90 (MQ=255) ctttAGTGGGGATCAGGTTTTTTTTACAACTGTTT‑GACAAGATAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg < 2:300580/88‑1 (MQ=255) aGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACaa > 2:545141/1‑90 (MQ=255) agcAGGTTTTTTTTAAAACTTTTT‑GAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc < 2:301987/88‑1 (MQ=255) aTCAGGTTTTTTTAACACTTTTTT‑GAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc < 2:457805/90‑1 (MQ=255) gagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGGAAGattat > 1:233955/1‑90 (MQ=255) | TCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTAT > NZ_CP009273/1819230‑1819400 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |