| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,962,939 | A→G | F228S (TTC→TCC) | cheR ← | protein‑glutamate O‑methyltransferase CheR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,962,939 | 0 | A | G | 90.9% | 57.0 / ‑1.4 | 22 | F228S (TTC→TCC) | cheR | protein‑glutamate O‑methyltransferase CheR |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (9/11); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCT > NZ_CP009273/1962858‑1963020 | tCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCa > 1:452896/1‑90 (MQ=255) cGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCgg < 2:332699/90‑1 (MQ=255) gAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCGGCa < 1:398469/90‑1 (MQ=255) aaCAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCGGCAc < 2:451844/90‑1 (MQ=255) aaCAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCGGCAc < 2:438615/90‑1 (MQ=255) aaCAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCGGCAc < 2:221017/90‑1 (MQ=255) gcgcAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCGGCAc < 2:508482/81‑1 (MQ=255) gcgcAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCGGCAc > 1:508482/1‑81 (MQ=255) aaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGc < 1:338436/90‑1 (MQ=255) aGTTTGATCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCgg < 2:645085/57‑1 (MQ=255) aGTTTGATCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCgg > 1:645085/1‑57 (MQ=255) tGATCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCa < 2:446479/90‑1 (MQ=255) tGATCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCa < 1:334509/90‑1 (MQ=255) aTCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCGGCAc > 2:274611/1‑54 (MQ=255) aTCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCGGCAc < 1:274611/54‑1 (MQ=255) aTCGAAGTAGATCATGACGTTACGACAGGAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaa > 2:297476/1‑90 (MQ=255) tGACGTTACGACAGGAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCg > 2:186119/1‑90 (MQ=255) aCGTTACGACAGGAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGcc > 2:305441/1‑90 (MQ=255) aCGTTACGACAGGAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGcc > 1:155434/1‑90 (MQ=255) cGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCa < 2:174482/90‑1 (MQ=255) ttACGACAGGAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGc > 1:395438/1‑90 (MQ=255) tACGACAGGAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCt > 2:637434/1‑90 (MQ=255) | TCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCT > NZ_CP009273/1962858‑1963020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |