| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,172,868 | G→C | G190A (GGC→GCC) | yegT → | nucleoside permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,172,868 | 0 | G | C | 96.0% | 77.7 / ‑4.5 | 25 | G190A (GGC→GCC) | yegT | nucleoside permease |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base C (17/7); total (18/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTTTCTTCTGTT > NZ_CP009273/2172784‑2172951 | tCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCaaaa < 2:661702/90‑1 (MQ=255) ccGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATg > 1:695394/1‑90 (MQ=255) ccgccgGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCa < 1:115294/90‑1 (MQ=255) gccgGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATg > 2:266764/1‑90 (MQ=255) cgGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCt < 2:347962/90‑1 (MQ=255) ggAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTc < 1:179801/90‑1 (MQ=255) ggAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTc < 2:202155/90‑1 (MQ=255) gctcGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCt > 1:232091/1‑90 (MQ=255) gctcGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCt > 1:53492/1‑90 (MQ=255) gctcGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCt > 1:58160/1‑90 (MQ=255) gctcGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCt > 2:19699/1‑90 (MQ=255) gctcGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCt > 1:421760/1‑90 (MQ=255) gtgtgtTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATcc > 1:389775/1‑90 (MQ=255) gtgtgtTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATcc > 2:350307/1‑90 (MQ=255) tttGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctgctg > 1:429390/1‑90 (MQ=255) gTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGa > 1:64240/1‑90 (MQ=255) gTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGa > 1:448955/1‑90 (MQ=255) gTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGa < 1:700958/90‑1 (MQ=255) gTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGa > 2:604625/1‑90 (MQ=255) gCCCGACACGCCACCAAAAAGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACtt > 1:556168/1‑90 (MQ=255) aaaaaGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTttctt > 2:659459/1‑90 (MQ=255) aaGCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTTTCTTCTg > 2:485056/1‑90 (MQ=255) gCACCGCCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTTTCTTCTGtt > 1:595081/1‑90 (MQ=255) cACCGCCAAAATGGATATTAAAGTCATGCTCGGCCt > 1:707561/1‑36 (MQ=38) cACCGCCAAAATGGATATTAAAGTCATGCTCGGCCt < 2:707561/36‑1 (MQ=38) | TCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTTTCTTCTGTT > NZ_CP009273/2172784‑2172951 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |