Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,189,312	A→G	T512A (ACC→GCC)	metG →	methionine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,189,312	0	A	G	100.0%	83.4 / NA	26	T512A (ACC→GCC)	metG	methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/15);  total (11/15)

CTGTTCCGCGTGCTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAG  >  NZ_CP009273/2189228‑2189395                                                                                     |                                                                                    cTGTTCCGCGTGCTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTgg                                                                                >  2:380287/1‑90 (MQ=255)   gTTCCGCGTGCTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGa                                                                              >  1:150908/1‑90 (MQ=255)      ccGCGTGCTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATgg                                                                           >  2:43047/1‑90 (MQ=255)        gcgTGCTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTa                                                                         >  2:253278/1‑90 (MQ=255)                  gaCTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAAcc                                                               <  2:505635/90‑1 (MQ=255)                      tACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCgctg                                                           >  1:310149/1‑90 (MQ=255)                               ccGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCcac                                                  <  2:324905/90‑1 (MQ=255)                               ccGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCcac                                                  <  1:576105/90‑1 (MQ=255)                               ccGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCcac                                                  <  1:688591/90‑1 (MQ=255)                                 ggTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACaa                                                >  2:195235/1‑90 (MQ=255)                                         cGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCGCAAAGTGAATc                                        <  1:390965/90‑1 (MQ=255)                                            aaCTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGt                                     >  2:195236/1‑90 (MQ=255)                                            aaCTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGt                                     >  1:561135/1‑90 (MQ=255)                                            aaCTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGAAACCGCTGCTGGGCCACAAAGTGAATCCGt                                     >  2:693673/1‑90 (MQ=255)                                                 aCCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGt                                                                          >  1:399369/1‑48 (MQ=255)                                                 aCCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGt                                                                          <  2:399369/48‑1 (MQ=255)                                                   cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc                              <  1:309982/90‑1 (MQ=255)                                                   cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc                              <  1:270323/90‑1 (MQ=255)                                                     aGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgcgc                            <  1:693673/90‑1 (MQ=255)                                                     aGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgcgc                            <  1:43047/90‑1 (MQ=255)                                                     aGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgcgc                            <  1:383524/90‑1 (MQ=255)                                                                  aTTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCAt               >  2:275118/1‑90 (MQ=255)                                                                   ttCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATc              <  1:625917/90‑1 (MQ=255)                                                                   ttCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATc              <  1:380287/90‑1 (MQ=255)                                                                             cGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGc    <  1:253278/90‑1 (MQ=255)                                                                               gAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAg  <  2:561135/90‑1 (MQ=255)                                                                                     |                                                                                    CTGTTCCGCGTGCTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAG  >  NZ_CP009273/2189228‑2189395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑