Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,505,777	T→C	I137V (ATC→GTC)	mntH ←	Nramp family divalent metal transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,505,777	0	T	C	100.0%	16.7 / NA	7	I137V (ATC→GTC)	mntH	Nramp family divalent metal transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/6);  total (1/6)

CGGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGT  >  NZ_CP009273/2505690‑2505860                                                                                        |                                                                                    cgGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGAccc                                                                                   <  1:48439/90‑1 (MQ=255) cgGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGAccc                                                                                   <  1:633601/90‑1 (MQ=255) cgGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGAccc                                                                                   <  2:614790/90‑1 (MQ=255) cgGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGAccc                                                                                   >  2:75347/1‑90 (MQ=255)              caGTACCCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACcgc                                                                      <  2:586076/90‑1 (MQ=255)                                    tcCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCa                                                <  2:421991/90‑1 (MQ=255)                                             ttttGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGAccc                                                                                   >  1:233457/1‑46 (MQ=255)                                             ttttGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGAccc                                                                                   <  2:233457/46‑1 (MQ=255)                                             ttttGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGt                                       >  2:131919/1‑90 (MQ=255)                                                              cagcaTTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATcgccgc                      <  2:660493/90‑1 (MQ=255)                                                                gcaTTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCAc                    <  1:296884/90‑1 (MQ=255)                                                                    tAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAAt                <  1:131919/90‑1 (MQ=255)                                                                                  tCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGt  <  2:410365/90‑1 (MQ=255)                                                                                        |                                                                                    CGGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGT  >  NZ_CP009273/2505690‑2505860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑