Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,801,112	A→C	T208P (ACC→CCC)	proX →	glycine betaine/L‑proline ABC transporter substrate‑binding protein ProX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,801,112	0	A	C	100.0%	67.3 / NA	21	T208P (ACC→CCC)	proX	glycine betaine/L‑proline ABC transporter substrate‑binding protein ProX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/10);  total (11/10)

CGTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCA  >  NZ_CP009273/2801027‑2801173                                                                                      |                                                              cGTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTg                                                           <  2:171749/90‑1 (MQ=255) cGTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTg                                                           <  1:49133/90‑1 (MQ=255)  gTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTgg                                                          <  1:187538/90‑1 (MQ=255)  gTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTgg                                                          >  2:431180/1‑90 (MQ=255)  gTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTgg                                                          >  2:187538/1‑90 (MQ=255)  gTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTgg                                                          >  1:514077/1‑90 (MQ=255)  gTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTgg                                                          >  1:55217/1‑90 (MQ=255)       gCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGcc                                                     >  2:575886/1‑90 (MQ=255)          tAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTa                                                  >  2:668880/1‑90 (MQ=255)                 gggAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTg                                           <  1:174190/90‑1 (MQ=255)                          gCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGc                                                      <  2:367561/70‑1 (MQ=255)                          gCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGc                                                      >  1:367561/1‑70 (MQ=255)                                 tgatgGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGc                           <  2:612714/90‑1 (MQ=255)                                 tgatgGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTCTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGc                           <  1:448482/90‑1 (MQ=255)                                              cATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTg                                               >  1:472110/1‑57 (MQ=255)                                              cATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTg                                               <  2:472110/57‑1 (MQ=255)                                                tCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATgtcg            >  1:475830/1‑90 (MQ=255)                                                     cGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTAc                                                 >  1:640719/1‑48 (MQ=255)                                                     cGCTACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTAc                                                 <  2:640719/48‑1 (MQ=255)                                                        tACAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTg    >  2:154405/1‑90 (MQ=255)                                                          cAAAGAGGGCAAACCGGTGTTTTATTACCCCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCa  <  1:668880/90‑1 (MQ=255)                                                                                      |                                                              CGTGACGCATAATCAGGGGAACTACGCAGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTATTACACCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCA  >  NZ_CP009273/2801027‑2801173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑