| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,849,315 | A→C | Y540S (TAC→TCC) | flhA → | formate hydrogenlyase transcriptional activator FlhA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,849,315 | 0 | A | C | 100.0% | 60.5 / NA | 20 | Y540S (TAC→TCC) | flhA | formate hydrogenlyase transcriptional activator FlhA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (7/13); total (7/13) | |||||||||||
GACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCGCTGCTGGCGAAAGCCTTT > NZ_CP009273/2849232‑2849394 | gacggacgTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTCCCGCCt < 1:73979/90‑1 (MQ=255) acggacgTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTCCCGCCTg < 1:114180/90‑1 (MQ=255) acggacgTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTCCCGCCTg < 1:723473/90‑1 (MQ=255) acgTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTCCCGCCTGAACg > 2:208142/1‑90 (MQ=255) gCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTCCCGCCTGAACGTAtt > 1:487723/1‑90 (MQ=255) cGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGGGTTCCGTAGCGATCTCTATTCCCGCCTGAACGTATTc < 2:391916/90‑1 (MQ=255) tCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTCCCGCCTGAACGTATTccc < 2:376512/90‑1 (MQ=255) aCTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTCCCGCCTGAACGTATTCCCGATTCACCTgccg < 2:59944/90‑1 (MQ=255) tacccGCGATCTAAAAAAAAGGGTCCCCGCCCGGGAGTCCCGTAGCGATCTCTATCCCCGCCTGAACGTATTCCCGATTCCCCTgccgcc < 1:675525/87‑1 (MQ=255) cccGCGATCTAAAAAAAATGGCCGCCGCCCGTGATTCCCGTAGCGATCTCTTTCCCCGCCTGAACGTATTCCCGATTCCCCTGCCGCCAc < 1:429900/89‑1 (MQ=255) aTCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTCCCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTAcgcg > 2:463260/1‑90 (MQ=255) cTGAAAAAATTGGTCGCCGACCGTGAGTTCCGTACCGATCTCTATTCCCGCCCGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAg < 1:587044/90‑1 (MQ=255) tGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTCCCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGc < 2:269044/90‑1 (MQ=255) gAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTCCCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCg > 1:431311/1‑90 (MQ=255) aaaaTGGTCGCCGCCCGTGAGTTCCGTAGCGATCTCTTTTCCCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCGTCCg < 1:201924/90‑1 (MQ=255) gTGAGTTCCGTAGCGATCTCTATTCCCGCCTGAACGTATTCCCGATTCACCTgccgcc > 1:391634/1‑58 (MQ=255) gTGAGTTCCGTAGCGATCTCTATTCCCGCCTGAACGTATTCCCGATTCACCTgccgcc < 2:391634/58‑1 (MQ=255) tCCGTAGCGATCTCTATTCCCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCGCTGCTGGCGa > 2:647372/1‑90 (MQ=255) gATCTCTATTCCCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCGCTGCTGGCGAAAGCCttt > 1:164869/1‑90 (MQ=255) gATCTCTATCCCCGCCTGAACGTTTCCCCGATTCCCCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCGCTGCTGGCGAAAGCCttt < 1:681056/90‑1 (MQ=38) | GACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCGCTGCTGGCGAAAGCCTTT > NZ_CP009273/2849232‑2849394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |