| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,940,645 | C→T | intergenic (‑108/‑101) | mltA ← / → BW25113_RS14635 | murein transglycosylase A/tRNA‑Met |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,940,645 | 0 | C | T | 85.7% | 72.7 / 3.6 | 28 | intergenic (‑108/‑101) | mltA/BW25113_RS14635 | murein transglycosylase A/tRNA‑Met |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base T (7/17); total (8/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01 | |||||||||||
TAAGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAG > NZ_CP009273/2940562‑2940726 | tAAGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGTTTTAGt > 2:374518/1‑90 (MQ=255) aaGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGTTTTAGTg > 1:557183/1‑90 (MQ=255) gcgTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCaa < 2:445300/90‑1 (MQ=255) cgTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCaaa < 2:327518/90‑1 (MQ=255) gTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAAt > 2:54503/1‑90 (MQ=255) aaatgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATc < 1:58623/90‑1 (MQ=255) gcagcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTa < 1:140325/90‑1 (MQ=255) cccTGACGCAAATTTTGCACAAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAgg > 1:507859/1‑90 (MQ=255) gACGCAAATTTTGCACAAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGc > 2:86324/1‑90 (MQ=255) gACGCAAATTTTGCACAAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGc > 2:370477/1‑90 (MQ=255) gACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGc > 2:91920/1‑90 (MQ=255) cAAATTTTGCACAAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGcc > 2:562029/1‑55 (MQ=255) cAAATTTTGCACAAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGcc < 1:562029/55‑1 (MQ=255) ttGCACAAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAAcgc < 2:553425/90‑1 (MQ=255) tGCACAAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAAcgcg < 1:192570/90‑1 (MQ=255) tGCACAAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAAcgcg < 2:40750/90‑1 (MQ=255) cacaAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAg < 2:501645/90‑1 (MQ=255) cacaAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAg < 2:557183/90‑1 (MQ=255) cacaAAAAATAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAg < 1:461345/90‑1 (MQ=255) caAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCg < 2:317572/90‑1 (MQ=255) aaaaaaTAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCgg < 2:372462/90‑1 (MQ=255) aaaaaaTAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCgg < 2:138528/90‑1 (MQ=255) aaaaaaTAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCgg < 1:523064/90‑1 (MQ=255) aaaaaaTAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCgg < 1:363158/90‑1 (MQ=255) aaaaaaTAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCgg < 2:218623/90‑1 (MQ=255) aaaaaaTAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCgg < 1:91920/90‑1 (MQ=255) aaaaTAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAg < 2:108663/90‑1 (MQ=255) aaaaTAGGTTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAg < 1:208078/90‑1 (MQ=255) | TAAGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAG > NZ_CP009273/2940562‑2940726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |