Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,579,168	T→C	E339G (GAA→GGA)	ggt ←	gamma‑glutamyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,579,168	0	T	C	100.0%	101.6 / NA	31	E339G (GAA→GGA)	ggt	gamma‑glutamyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/15);  total (16/15)

GATCGGCAATAGATTTGGCATAGGCTTTATTGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATTCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTATTTCTT  >  NZ_CP009273/3579088‑3579247                                                                                 |                                                                                gATCGGCAATAGATTTGGCATAGGCTTTATTGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGt                                                                        >  2:88524/1‑90 (MQ=255)             aTTTGGCATAGGCTTTATTGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCggcgtaggcgt                                                            <  1:519462/90‑1 (MQ=255)                tGGCATAGGCTTTATTGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTAtt                                                         >  2:581559/1‑90 (MQ=255)                tGGCATAGGCTTTATTGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTAtt                                                         >  1:648310/1‑90 (MQ=255)                      aGGCTTTATTGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCg                                                   >  1:90304/1‑90 (MQ=255)                        gCTTTATTGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCt                                                 <  2:253578/90‑1 (MQ=255)                        gCTTTATTGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCt                                                 <  2:722372/90‑1 (MQ=255)                          tttATTGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTc                                               <  2:596087/90‑1 (MQ=255)                          tttATTGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTc                                               <  2:592505/90‑1 (MQ=255)                           ttATTGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCt                                              >  1:582403/1‑90 (MQ=255)                           ttATTGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGGCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCt                                              >  1:490140/1‑90 (MQ=255)                               tGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCa                                          <  1:88524/90‑1 (MQ=255)                                   cAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGAt                                      >  2:591485/1‑90 (MQ=255)                                     gcgcCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGAttt                                    >  1:31309/1‑90 (MQ=255)                                     gcgcCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGAttt                                    >  2:142772/1‑90 (MQ=255)                                       gcCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTgc                                  >  1:229699/1‑90 (MQ=255)                                         cTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTgcat                                <  2:363841/90‑1 (MQ=255)                                          tGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTgcatc                               <  2:211917/90‑1 (MQ=255)                                          tGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGCAGGCGTATTTCTCCGCTTCTGCCATGATTTgcatc                               >  2:40147/1‑90 (MQ=255)                                               cGGTACTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTgcatcgcatc                          >  1:472142/1‑90 (MQ=255)                                                   aCTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGCAGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGgcg                      <  1:40147/90‑1 (MQ=255)                                                    cTTTGACAAAATCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGgcgc                     >  2:272566/1‑90 (MQ=255)                                                           aaaaTCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCaaa              <  2:270256/90‑1 (MQ=255)                                                           aaaaTCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCaaa              <  2:648310/90‑1 (MQ=255)                                                             aaTCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGc            <  2:551760/90‑1 (MQ=255)                                                             aaTCCGGGTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGc            <  1:548787/90‑1 (MQ=255)                                                                  gggTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTAt       >  2:58956/1‑90 (MQ=255)                                                                  gggTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTAt       >  2:179723/1‑90 (MQ=255)                                                                  gggTCGCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTAt       <  1:693605/90‑1 (MQ=255)                                                                       gCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTATttctt  <  2:490140/90‑1 (MQ=255)                                                                       gCCAAGATATCCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTATttctt  >  2:619043/1‑90 (MQ=255)                                                                                 |                                                                                GATCGGCAATAGATTTGGCATAGGCTTTATTGGTCAGCGCCTGCCACGGTACTTTGACAAAATCCGGGTCGCCAAGATATTCCGAGCGGTCGGCGTAGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTATTTCTT  >  NZ_CP009273/3579088‑3579247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑