| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,649,723 | G→C | intergenic (‑71/+45) | hdeB ← / ← hdeA | acid‑activated periplasmic chaperone HdeB/acid‑activated periplasmic chaperone HdeA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,649,723 | 0 | G | C | 96.8% | 95.9 / ‑3.2 | 31 | intergenic (‑71/+45) | hdeB/hdeA | acid‑activated periplasmic chaperone HdeB/acid‑activated periplasmic chaperone HdeA |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (15/15); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01 | |||||||||||
AGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTAATTTTGTCCCATTCGCCTTTAACTTT > NZ_CP009273/3649636‑3649809 | agagaTGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGccc < 2:606969/90‑1 (MQ=255)agagaTGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGccc < 2:554714/90‑1 (MQ=255)agagaTGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGccc > 1:414338/1‑90 (MQ=255)agaaaTGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGccc < 2:323543/90‑1 (MQ=255) gggaTGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCt < 1:340035/88‑1 (MQ=255) agaTGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTg > 2:700369/1‑90 (MQ=255) gaTGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGt < 2:522170/90‑1 (MQ=255) tGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTc < 2:166749/90‑1 (MQ=255) aaaTATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCaa < 2:253773/90‑1 (MQ=255) ttCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAGGATATTCCGCCCTGTTCAATATGc < 2:537148/90‑1 (MQ=255) cATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCaa < 1:213286/90‑1 (MQ=255) cATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCaa < 2:605299/90‑1 (MQ=255) cATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCaa < 1:700369/90‑1 (MQ=255) tAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTa < 1:45034/90‑1 (MQ=255) aaCCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTAc < 2:272849/90‑1 (MQ=255) cATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATg > 1:419475/1‑90 (MQ=255) ttCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTa > 1:77833/1‑90 (MQ=255) gagaTTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTcc > 2:159576/1‑90 (MQ=255) agaTTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTccc < 2:77833/90‑1 (MQ=255) agaTTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTccc < 1:675702/90‑1 (MQ=255) agaTTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTccc < 1:240235/90‑1 (MQ=255) tatataTGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGaa > 2:266323/1‑90 (MQ=255) tatataTGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGaa > 2:407766/1‑90 (MQ=255) tatataTGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGaa > 1:422982/1‑90 (MQ=255) tatataTGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGaa > 1:374898/1‑90 (MQ=255) tatataTGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGaa > 1:130138/1‑90 (MQ=255) gAACAGGAGGATTAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACata > 1:488056/1‑90 (MQ=255) gAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACata > 2:603653/1‑90 (MQ=255) aaTAGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTaa > 1:246519/1‑90 (MQ=255) aGCTGCTTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTAAttt < 1:445818/90‑1 (MQ=255) gctTTTAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTAATTTTGTc > 1:255104/1‑90 (MQ=255) ttttAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTAATTTTGTccc > 1:653829/1‑90 (MQ=255) ttttAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTAATTTTGTccc > 1:184649/1‑90 (MQ=255) tAAAGAAGATATTCCGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTAATTTTGTCCCAtt < 2:579170/90‑1 (MQ=255) ccGCCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTAATTTTGTCCCATTCGCCTTTAACttt < 2:255104/90‑1 (MQ=255) | AGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTAATTTTGTCCCATTCGCCTTTAACTTT > NZ_CP009273/3649636‑3649809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |