Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,786,991	T→C	D18G (GAT→GGT)	yibB ←	protein YibB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,786,991	0	T	C	100.0%	59.0 / NA	19	D18G (GAT→GGT)	yibB	protein YibB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/12);  total (7/12)

AAGTGCGGCCAGACGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAATCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGGATGATTTCATTACTAATAAGACCATTAATATAAAAAAAGAA  >  NZ_CP009273/3786906‑3787074                                                                                      |                                                                                    aaGTGCGGCCAGACGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACcccc                                                                                 <  2:580095/90‑1 (MQ=255)  aGTGCGGCCAGACGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCct                                                                                <  2:653691/90‑1 (MQ=255)    tGCGGCCAGACGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCctct                                                                              >  2:62165/1‑90 (MQ=255)     gCGGCCAGACGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTa                                                                             >  1:416668/1‑90 (MQ=255)              cGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGGTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCa                                                                    <  1:648273/90‑1 (MQ=255)                      gTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGc                                                            <  1:716118/90‑1 (MQ=255)                      gTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGc                                                            <  2:588294/90‑1 (MQ=255)                       tAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCa                                                           <  1:239389/90‑1 (MQ=255)                       tAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCa                                                           <  1:312119/90‑1 (MQ=255)                       tAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCa                                                           <  2:680951/90‑1 (MQ=255)                          cTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCAGTg                                                        <  1:227665/90‑1 (MQ=255)                                              aCGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGGATGAttt                                    >  1:195851/1‑90 (MQ=255)                                                 aGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGGATGATTTCAt                                 >  1:26130/1‑90 (MQ=255)                                                 aGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGGATGATTTCAt                                 <  1:599478/90‑1 (MQ=255)                                                 aGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGGATGATTTCAt                                 <  2:26130/90‑1 (MQ=255)                                                 aGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGGCAAAATATGCa                                                           <  1:685299/64‑1 (MQ=255)                                                 aGCTAGTTTTTCACGGAACCCTTTATTAGCAGGCCAACCCCCTCTACCAATGTCAAAATATGCa                                                           >  2:685299/1‑64 (MQ=255)                                                      gTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGGATGATTTCATTACta                            >  1:169295/1‑90 (MQ=255)                                                                                gTCCAACCCCCTCTACCAATGTCAAAATATGCAGCGATAATTGTGGTGGATGATTTCATTACTAATAAGACCATTAATATAAAAAAAGaa  >  2:585350/1‑90 (MQ=255)                                                                                      |                                                                                    AAGTGCGGCCAGACGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAATCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGGATGATTTCATTACTAATAAGACCATTAATATAAAAAAAGAA  >  NZ_CP009273/3786906‑3787074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑