Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,903	T→A	D27E (GAT→GAA)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,903	0	T	A	100.0%	38.2 / NA	13	D27E (GAT→GAA)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (9/4);  total (9/4)

TCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGT  >  NZ_CP009273/49827‑49982                                                                             |                                                                                tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTa                                                                    <  1:159509/90‑1 (MQ=255)    gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGGACCTGCCTGCCGAACTCGCCTGGTTTAAAc                                                                 >  1:375417/1‑90 (MQ=255)        gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                             <  2:90178/90‑1 (MQ=255)         aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAac                                                            >  1:160263/1‑90 (MQ=255)              ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt                                                       >  1:13310/1‑90 (MQ=255)                                  ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                   >  1:270995/1‑90 (MQ=255)                                          aTGGAAACCGCCAGGCCGTGAACCCGCCCTCCCGAACCCGCCCGGTTTAAACGCAACCCCTTAAATAACCCCGTGATTATGGACCGCCAt                           <  1:465327/90‑1 (MQ=255)                                          aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                           >  2:716996/1‑90 (MQ=255)                                                  cGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                   >  1:22412/1‑90 (MQ=255)                                                  cGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                   <  1:271916/90‑1 (MQ=255)                                                       tGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGGGATCATGGGCTTCCATACNTNTNnatcaa              >  1:343185/1‑90 (MQ=255)                                                           gTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg          >  2:157400/1‑90 (MQ=255)                                                                   tgcctgccGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt  >  2:47038/1‑90 (MQ=255)                                                                             |                                                                                TCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGT  >  NZ_CP009273/49827‑49982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑