| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,527,151 | T→C | Q87Q (CAA→CAG) | nanS ← | 9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,527,151 | 0 | T | C | 97.8% | 147.9 / NA | 45 | Q87Q (CAA→CAG) | nanS | 9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (23/21); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTTTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTAA > NZ_CP009273/4527068‑4527236 | aTAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCtgatga > 1:285057/1‑90 (MQ=255)aTAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCtgatga > 2:639451/1‑90 (MQ=255) aaTCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGt < 2:60591/90‑1 (MQ=255) cTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCa > 1:109141/1‑90 (MQ=255) cTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGGGTCTGATGATTCGTTGCCa > 2:423149/1‑90 (MQ=255) tGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCag < 1:588627/90‑1 (MQ=255) ttATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa < 1:124200/90‑1 (MQ=255) ttATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa < 2:436159/90‑1 (MQ=255) ttATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa < 2:55804/90‑1 (MQ=255) ttATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGGGCCTGGCCAACGGTTCCGTACTGTATCTGATGATTCGTTGCCagagta < 1:388722/90‑3 (MQ=255) aTCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg < 1:632804/90‑1 (MQ=255) aTCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg < 1:139807/90‑1 (MQ=255) gAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGAt < 1:120086/90‑1 (MQ=255) gAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGAt < 2:653492/90‑1 (MQ=255) aTGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATaa > 1:415978/1‑90 (MQ=255) aaGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa > 1:416712/1‑78 (MQ=255) aaGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa < 2:416712/78‑1 (MQ=255) gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa < 2:621805/90‑1 (MQ=255) gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa > 2:126129/1‑90 (MQ=255) aGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGAAAACCCTGCata < 2:346481/90‑1 (MQ=255) aTTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCt < 1:409673/90‑1 (MQ=255) ttcagTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGa > 2:76089/5‑90 (MQ=255) cGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACa < 1:568504/90‑1 (MQ=255) atatGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCgtgt < 1:685387/90‑1 (MQ=255) cAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCa > 2:352675/1‑90 (MQ=255) gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg < 2:55655/52‑1 (MQ=255) gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg > 1:55655/1‑52 (MQ=255) gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCt > 1:700649/1‑72 (MQ=255) gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCt < 2:700649/72‑1 (MQ=255) gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGt > 1:131054/1‑90 (MQ=255) tGGCCAACGGTGCCGTACTGTGTTTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTc < 1:379102/90‑1 (MQ=255) tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGa > 1:545857/1‑87 (MQ=255) tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGa < 2:545857/87‑1 (MQ=255) tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTc > 2:452451/1‑90 (MQ=255) tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTc > 1:444104/1‑90 (MQ=255) tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTc < 1:415236/90‑1 (MQ=255) tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTc > 1:57324/1‑90 (MQ=255) aaCGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCgtgt < 2:425850/73‑1 (MQ=255) aaCGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCgtgt > 1:425850/1‑73 (MQ=255) gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa > 2:382919/1‑90 (MQ=255) gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa > 1:486265/1‑90 (MQ=255) gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa > 2:288832/1‑90 (MQ=255) gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa > 2:579485/1‑90 (MQ=255) gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa > 1:323197/1‑90 (MQ=255) gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa > 2:267393/1‑90 (MQ=255) | ATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTTTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTAA > NZ_CP009273/4527068‑4527236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |