| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 883,127 | C→G | G83G (GGC→GGG) | rcdA → | DNA‑binding transcriptional regulator RcdA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 883,127 | 0 | C | G | 90.0% | 56.8 / 0.6 | 20 | G83G (GGC→GGG) | rcdA | DNA‑binding transcriptional regulator RcdA |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base G (7/11); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
TGTTACTGGAGGCGTTCAGCAGTTTTACTGAGATCATGTCCCGGCAATATCAGGCATTTTTTAGCGATGTTAGTGATGCTCCGGGCGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAACGCCGGATAACATGGAGCTGATGTACCAGCTCTACGCGC > NZ_CP009273/883042‑883212 | tgttACTGGAGGCGTTCAGCAGTTTTACTGAGATCATGTCCCGGCAATATCAGGCATTTTTTAGCGATGTTAGTGATGCTCCGGGGGCAt > 1:210909/1‑90 (MQ=255) aGGCGTTCAGCAGTTTTACTGAGATCATGTCCCGGCAATATCAGGCATTTTTTAGCGATGTTAGTGATGCTCCGGGGGCATGCCAGGCTa < 2:51422/90‑1 (MQ=255) cagTTTTACTGAGATCATGTCCCGGCAATATCAGGCATTTTTTAGCGATGTTAGTGATGCTCCGGGGGCATGCCAGGCTATCACCGatat < 2:293703/90‑1 (MQ=255) ttACTGAGATCATGTCCCGGCAATATCAGGCATTTTTTAGCGATGTTAGGGATGCTCCGGGGGCATGCCAGGCTATCACCGATATGATCt > 1:702328/1‑90 (MQ=255) gaTCATGTCCCGGCAATATCAGGCATTTTTTAGCGATGTTAGTGATGCTCCGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTc > 2:656334/1‑90 (MQ=255) gaTCATGTCCCGGCAATATCAGGCATTTTTTAGCGATGTTAGTGATGCTCCGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTc > 1:494909/1‑90 (MQ=255) cGGCAATATCAGGCATTTTTTAGCGATGTTAGTGATGCTCCGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCa > 2:425517/1‑90 (MQ=255) gCAATATCAGGCATTTTTTAGCGATGTTAGTGATGCTCCGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAAc < 2:168717/90‑1 (MQ=255) aaTATCAGGCATTTTTTAGCGATGTTAGTGATGCTCCGGGCGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAACGc < 2:182523/90‑1 (MQ=255) tatCAGGCATTTTTTAGCGATGTTAGTGATGCTCCGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAACGCCg < 2:210909/90‑1 (MQ=255) cAGGCATTTTTTAGCGATGTTAGTGATGCTCCGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTCACCGGTTGCAACGCCGGAt < 2:95942/90‑1 (MQ=255) aTTTTTTAGCGATGTTAGTGATGCTCCGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAACGCCGGATAACAt < 2:39105/90‑1 (MQ=255) aTTTTTTAGCGATGTTAGTGATGCTCCGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAACGCCGGATAACAt < 2:432244/90‑1 (MQ=255) ttttttAGCGATGTTAGTGATGCTCCGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAACGCCGGATAACATg > 1:500050/1‑90 (MQ=255) tttAGCGATGTTAGTGATGCTCCGGGCGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAACGCCGGATAACATGGAg < 1:154516/90‑1 (MQ=255) ttAGCGATTTTAGTGATGCTCCGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAACGCCGGATAACATGGAGc < 1:83422/90‑1 (MQ=255) cGATGTTAGTGATGCTCCGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAACGCCGGATAACATGGAGCTGAt < 1:221708/90‑1 (MQ=255) cGATGTTAGTGATGCTCCGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAACGCCGGATAACATGGAGCTGAt < 2:554508/90‑1 (MQ=255) cTCCGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAACGCCGGATAACATGGAGCTGATGTACCAGCTCTAcg > 2:421708/1‑90 (MQ=255) cGGGGGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAACGCCGGATAACATGGAGCTGATGTACCAGCTCTACgcgc < 1:143901/90‑1 (MQ=255) | TGTTACTGGAGGCGTTCAGCAGTTTTACTGAGATCATGTCCCGGCAATATCAGGCATTTTTTAGCGATGTTAGTGATGCTCCGGGCGCATGCCAGGCTATCACCGATATGATCTACAGCTCACAGGTTGCAACGCCGGATAACATGGAGCTGATGTACCAGCTCTACGCGC > NZ_CP009273/883042‑883212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |