Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	886,189	A→G	G7G (GGT→GGC)	grxA ←	glutaredoxin 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	886,189	0	A	G	100.0%	57.2 / NA	18	G7G (GGT→GGC)	grxA	glutaredoxin 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/9);  total (9/9)

ACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGAAA  >  NZ_CP009273/886103‑886268                                                                                       |                                                                                aCATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCa                                                                              <  1:260402/90‑1 (MQ=255)    tactgatactgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCaaaa                                                                           >  2:252534/1‑90 (MQ=255)      ctgatactgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAAt                                                                         >  1:263806/1‑90 (MQ=255)              gaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGttt                                                                 >  1:520380/1‑90 (MQ=255)                          gTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTtctc                                                     <  1:252534/90‑1 (MQ=255)                                 gCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCAt                                              <  2:60402/90‑1 (MQ=255)                                  cTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATa                                             <  2:627935/90‑1 (MQ=255)                                          ctcAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATg                                     <  2:692984/90‑1 (MQ=255)                                          ctcAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATg                                     <  2:205947/90‑1 (MQ=255)                                                       tttGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCa                        <  1:427499/90‑1 (MQ=255)                                                                         gggCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCt      >  2:512266/1‑90 (MQ=255)                                                                             aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATa                                             >  2:359902/1‑47 (MQ=255)                                                                             aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATa                                             <  1:359902/47‑1 (MQ=255)                                                                             aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGcc                                   <  2:30938/57‑1 (MQ=255)                                                                             aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGcc                                   >  1:30938/1‑57 (MQ=255)                                                                             aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGaaa  >  1:456647/1‑90 (MQ=255)                                                                             aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGaaa  >  2:283963/1‑90 (MQ=255)                                                                             aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGaaa  >  1:248356/1‑90 (MQ=255)                                                                                       |                                                                                ACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCACGCTAAAATGTATTCGCTGAAA  >  NZ_CP009273/886103‑886268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑