Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	987,365	A→G	N430D (AAT→GAT)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	987,365	0	A	G	100.0%	65.9 / NA	21	N430D (AAT→GAT)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/11);  total (10/11)

AAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCG  >  NZ_CP009273/987282‑987445                                                                                    |                                                                                 aaGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCg                                                                            <  2:649625/90‑1 (MQ=255)     gATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGAtct                                                                        <  2:586262/90‑1 (MQ=255)           gCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCa                                                                  >  2:179399/1‑90 (MQ=255)                     gAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGt                                                        <  1:297150/90‑1 (MQ=255)                     gAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGt                                                        >  1:340546/1‑90 (MQ=255)                     gAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCACTTCCGCCGt                                                        >  2:680548/1‑90 (MQ=255)                         gTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGt                                                    <  1:94600/90‑1 (MQ=255)                               atgGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGcc                                              <  2:340546/90‑1 (MQ=255)                                 ggTAGTGCAGCGACCTGTGACGATTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAg                                            <  1:111245/90‑1 (MQ=255)                                   tAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTc                                          >  1:329392/1‑90 (MQ=255)                                   tAGGGCCGCGCCCTGTGCCGTTTTTGGCCGGGGGATGAAAGAGGGGTGGGATGTCGACCCCCCCCATCCCCGCCGTTGGTACAGCCAGTc                                          <  1:497596/90‑1 (MQ=255)                                       gCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGt                                      >  2:424840/1‑90 (MQ=255)                                            gACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACAcc                                 >  2:554664/1‑90 (MQ=255)                                                    acgacTTTGTGCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGa                         <  1:554664/90‑1 (MQ=255)                                                           tgtgCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCaa                  >  1:664431/1‑90 (MQ=255)                                                           tgtgCAGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCaa                  <  1:423577/90‑1 (MQ=255)                                                                aGGCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAgacg             <  2:329392/90‑1 (MQ=255)                                                                 ggCGATGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAgacga            >  2:562222/1‑90 (MQ=255)                                                                      tGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCaa                  <  2:26286/79‑1 (MQ=255)                                                                      tGGAAGATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCaa                  >  1:26286/1‑79 (MQ=255)                                                                           gATGCGTCGGATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCg  >  2:485795/1‑90 (MQ=255)                                                                                    |                                                                                 AAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCG  >  NZ_CP009273/987282‑987445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑