Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	108,362	A→G	F242S (TTC→TCC)	zapD ←	cell division protein ZapD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	108,362	0	A	G	100.0%	67.7 / NA	21	F242S (TTC→TCC)	zapD	cell division protein ZapD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/8);  total (13/8)

CACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTT  >  NZ_CP009273/108281‑108446                                                                                  |                                                                                     cacaCCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGa                                                                              >  1:237783/1‑90 (MQ=255)  acacCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGAc                                                                             <  2:637784/90‑1 (MQ=255)  acacCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGAc                                                                             >  2:13255/1‑90 (MQ=255)   caccacCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACg                                                                            <  2:463659/90‑1 (MQ=255)       accGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTcc                                                                        <  1:329775/90‑1 (MQ=255)       accGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTcc                                                                        <  2:461503/90‑1 (MQ=255)            tttCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTAc                                                                   >  2:650615/1‑90 (MQ=255)                        tGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTc                                                       <  1:269119/90‑1 (MQ=255)                          ggCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAg                                                     <  1:355614/90‑1 (MQ=255)                                   ccGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCg                                            >  2:706278/1‑90 (MQ=255)                                        aTAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATa                                       >  2:132427/1‑90 (MQ=255)                                           gTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATaaaa                                    <  2:344443/90‑1 (MQ=255)                                             ttCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACg                                  >  2:109148/1‑90 (MQ=255)                                               cTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGaa                                >  1:344089/1‑90 (MQ=255)                                                     tttttACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCaa                          >  1:336272/1‑90 (MQ=255)                                                      ttttACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCaaa                         >  2:589487/1‑90 (MQ=255)                                                               ttAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCtt                >  1:683727/1‑90 (MQ=255)                                                                tAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTa               >  1:344457/1‑90 (MQ=255)                                                                       aGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGa                                 <  2:215737/65‑1 (MQ=255)                                                                       aGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGa                                 >  1:215737/1‑65 (MQ=255)                                                                             gTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAttt  >  2:239033/1‑90 (MQ=255)                                                                                  |                                                                                     CACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTT  >  NZ_CP009273/108281‑108446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑