| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,153,703 | A→T | I127F (ATC→TTC) | ptsG → | PTS glucose transporter subunit IIBC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,153,703 | 0 | A | T | 100.0% | 63.2 / NA | 18 | I127F (ATC→TTC) | ptsG | PTS glucose transporter subunit IIBC |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (8/10); total (8/10) | |||||||||||
TGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATC > NZ_CP009273/1153620‑1153789 | tGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAg > 1:613074/1‑90 (MQ=255) aTTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGAATATCTCCGGTGCGTTCGCAGCGTACATGt > 2:382040/1‑90 (MQ=255) aCCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGAATATCTCCGGTGCGTTCGCAGCGTACATGTTTaa < 1:382040/90‑1 (MQ=255) gccgaagaaATCGCCTCTAAACACCTGGGGGATAATGGCAAACTCGGAGGGATTGTCTCCGGGGGGTTCCCGGCGCACATGTTTATCCGt > 1:597846/4‑90 (MQ=255) gaagaaATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTc < 2:613074/90‑1 (MQ=255) gaagaaATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTc < 2:317176/90‑1 (MQ=255) ggCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGc > 2:701258/1‑45 (MQ=255) ggCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGc < 1:701258/45‑1 (MQ=255) ggCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTa > 1:371954/1‑90 (MQ=255) aCTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACa < 2:224737/38‑1 (MQ=255) aCTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACa > 1:224737/1‑38 (MQ=255) gggATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTaaa < 2:37963/90‑1 (MQ=255) ccGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGc < 1:351528/90‑1 (MQ=255) ggTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCg > 1:162193/1‑90 (MQ=255) ggTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCg > 1:493487/1‑90 (MQ=255) gCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATc < 2:29313/90‑1 (MQ=255) gCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATc < 2:371954/90‑1 (MQ=255) gCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATc < 2:493487/90‑1 (MQ=255) | TGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATC > NZ_CP009273/1153620‑1153789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |