Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,174,665	T→G	L206W (TTG→TGG)	nagK →	N‑acetylglucosamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,174,665	0	T	G	92.3%	35.6 / ‑3.1	13	L206W (TTG→TGG)	nagK	N‑acetylglucosamine kinase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (7/5);  total (8/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01

GCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTTGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTGGATTTATTA  >  NZ_CP009273/1174591‑1174747                                                                           |                                                                                   gCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTGGCAGGCTCCCGAAat                                                                     >  1:166862/1‑90 (MQ=255) gCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTGGCAGGCTCCCGAAat                                                                     >  2:572551/1‑90 (MQ=255)       cTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTGGCAGGCTCCCGAAATTATTgc                                                               <  1:287449/90‑1 (MQ=255)                    tATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCaa                                                  <  2:398873/90‑1 (MQ=255)                         gtctgGTCGCGGTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGa                                             <  1:338341/90‑1 (MQ=255)                              gTCGCGGTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGc                                        <  2:39300/90‑1 (MQ=255)                                          cGTGGCTGAATCAACACTATTATCATCAACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGgcgc                            <  1:281865/90‑1 (MQ=255)                                                      aaCACTATTATCATCAACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGtt                >  1:234994/1‑90 (MQ=255)                                                        cacTATTATCATCAACCGTTGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTAt              >  2:679372/1‑90 (MQ=255)                                                           tattatCATCAACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTg           >  1:290060/1‑90 (MQ=255)                                                                    caACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTGGATttatta  >  2:104766/1‑90 (MQ=255)                                                                    caACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTGGATttatta  >  2:282591/1‑90 (MQ=255)                                                                    caACCGTGGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTGGATttatta  >  2:591853/1‑90 (MQ=255)                                                                           |                                                                                   GCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACTATTATCATCAACCGTTGCAGGCTCCCGAAATTATTGCGCTTTATGATCAAGGCGATGAGCAGGCAAGGGCGCACGTTGAGCGTTATCTGGATTTATTA  >  NZ_CP009273/1174591‑1174747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑