Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,402,000 A→G I18T (ATC→ACC)  dgcM ← diguanylate cyclase DgcM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,402,0000AG95.7% 67.2 / ‑3.5 23I18T (ATC→ACC) dgcMdiguanylate cyclase DgcM
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (11/11);  total (11/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

GTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTATTTTTATCAATTTTTG  >  NZ_CP009273/1401913‑1402081
                                                                                       |                                                                                 
gTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTc                                                                                 <  1:10245/90‑1 (MQ=255)
gTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTc                                                                                 <  1:49846/90‑1 (MQ=255)
  tCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCa                                                                               >  1:455468/1‑90 (MQ=255)
   cGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAg                                                                              >  2:717636/1‑90 (MQ=255)
     tAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGAc                                                                            <  1:475064/90‑1 (MQ=255)
          gACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGAc                                                                       >  1:587360/1‑90 (MQ=255)
          gACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGAc                                                                       <  2:319583/90‑1 (MQ=255)
          gACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGAc                                                                       >  1:86172/1‑90 (MQ=255)
           aCTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCCCAAAGGGCGAAACGGTCCAGACAGGACt                                                                      <  2:165235/90‑1 (MQ=255)
              aaaCGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGt                                                                   <  1:46741/90‑1 (MQ=255)
                          ttGCATCAACAGTTTCGCCGCGCTATTGGCATCAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAg                                                       >  1:562689/1‑90 (MQ=255)
                          ttGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAg                                                       <  1:477258/90‑1 (MQ=255)
                          ttGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAg                                                       <  1:717636/90‑1 (MQ=255)
                          ttGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAg                                                       <  2:396925/90‑1 (MQ=255)
                          ttGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAg                                                       <  2:560870/90‑1 (MQ=255)
                                 aaCAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTg                                                >  2:697230/1‑90 (MQ=255)
                                 aaCAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGAATTg                                                >  1:584685/1‑90 (MQ=255)
                                     gTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGt                                            <  2:195502/90‑1 (MQ=255)
                                            cgcgCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGt                                     <  1:206921/90‑1 (MQ=255)
                                                                   gTTCCTCAAAGGGCGAAACGGTCCTGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTAtttt              >  2:222928/1‑90 (MQ=255)
                                                                   gTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTAtttt              >  2:316161/1‑90 (MQ=255)
                                                                     tCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTATTTTTa            >  1:576714/1‑90 (MQ=255)
                                                                               gCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTATTTTTATCAATTTttg  >  1:285714/1‑90 (MQ=255)
                                                                                       |                                                                                 
GTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTATTTTTATCAATTTTTG  >  NZ_CP009273/1401913‑1402081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑