Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,431,536	T→C	E1169G (GAA→GGA)	nifJ ←	pyruvate:ferredoxin (flavodoxin) oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,431,536	0	T	C	100.0%	67.1 / NA	21	E1169G (GAA→GGA)	nifJ	pyruvate:ferredoxin (flavodoxin) oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/8);  total (13/8)

ACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCC  >  NZ_CP009273/1431456‑1431616                                                                                 |                                                                                 aCAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTc                                                                         >  1:24451/1‑90 (MQ=255)              gtcgtcCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGc                                                            >  2:351397/1‑90 (MQ=255)                cgtcCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCa                                                          >  2:19189/1‑90 (MQ=255)                   ccGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGa                                                       <  1:180389/90‑1 (MQ=255)                          ttttttttAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATa                                                <  1:228454/90‑1 (MQ=255)                                             aTCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCt                             >  2:11705/1‑90 (MQ=255)                                               ccTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTgc                           <  2:24451/90‑1 (MQ=255)                                                     ccAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATc                     <  1:361667/90‑1 (MQ=255)                                                     ccAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATc                     <  1:574721/90‑1 (MQ=255)                                                       agagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCtt                   >  2:327967/1‑90 (MQ=255)                                                        gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATc                                 >  2:333957/1‑75 (MQ=255)                                                        gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATc                                 <  1:333957/75‑1 (MQ=255)                                                        gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCttt                  >  2:41419/1‑90 (MQ=255)                                                        gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCttt                  >  2:1678/1‑90 (MQ=255)                                                        gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCttt                  >  2:127699/1‑90 (MQ=255)                                                        gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCttt                  >  1:492530/1‑90 (MQ=255)                                                        gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCttt                  >  1:157016/1‑90 (MQ=255)                                                             tttAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATa             >  2:390345/1‑90 (MQ=255)                                                                    ggTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAATCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTc      >  1:110965/1‑90 (MQ=255)                                                                       gtTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGc   <  1:533774/90‑1 (MQ=255)                                                                        ttGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGcc  <  1:233684/90‑1 (MQ=255)                                                                                 |                                                                                 ACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCC  >  NZ_CP009273/1431456‑1431616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑