| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 115,628 | A→C | T137P (ACG→CCG) | ampD → | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 115,628 | 0 | A | C | 91.4% | 82.3 / ‑5.6 | 35 | T137P (ACG→CCG) | ampD | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (18/14); minor base T (3/0); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCC > NZ_CP009273/115542‑115714 | gCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCcgc < 2:6529/90‑1 (MQ=255) tGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGc < 1:395961/90‑1 (MQ=255) tttttCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACt < 2:309790/90‑1 (MQ=255) ttCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACtgat > 2:75651/1‑90 (MQ=255) tGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCCCTGATTGATTGCTATCCGGatat > 1:161734/1‑90 (MQ=255) tGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCCCGGGGCCTGATTGATTTCTATCCGGatat > 1:719497/1‑90 (MQ=255) gAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTTATTGCTATCCGGATATc > 1:28802/1‑90 (MQ=255) gCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTa < 2:407052/90‑1 (MQ=255) cACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTaa > 2:120931/1‑90 (MQ=255) aCCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTaaa > 2:163457/1‑90 (MQ=255) cGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAAGa > 2:643426/1‑90 (MQ=255) ggCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACggg > 2:82131/1‑90 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 2:651592/90‑1 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 1:229378/90‑1 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 2:183254/90‑1 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 2:200891/90‑1 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTTCGCGGGCCCTGATTGATTTCTATCCGGATATCCCTAAAAAAAAGACGGGCCCTTGTGat > 1:631404/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGATTGATTTCTATCCGGATATCGCTAAAAACATGACGGGCCCTTGTGat > 2:664382/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGATTGATTGCTATCCGGATATCGCTAAAAAAAATACGGGCCATTGTGat > 1:618724/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGat > 2:296495/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGat > 2:651669/1‑90 (MQ=255) cAGTATCAACAGCTTGCGGCGGTTTCGCGGGCACTGATTGATTGCTATCCCGATATTGCTAAAAAAAAGACGGGCCCTTTTGATAtttgg > 1:664677/1‑87 (MQ=255) aGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGGTATCGCTAAAAACATGACGGGCCATTGTGATATTtcgc > 2:537827/1‑90 (MQ=255) gTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGcc > 1:53785/1‑90 (MQ=255) cAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGAt < 1:105317/90‑1 (MQ=255) aGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGa < 1:680338/90‑1 (MQ=255) gCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGaa < 2:53785/90‑1 (MQ=255) cTTGCGGCGGTTTCGCGGGCACTGGTTGGGTGCTATCCGGGGATCGCTAAAAACAAGACGGGCCCTTTGGATATTGCGCCGGGTCGGaaa > 1:600926/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTTCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGaaa > 2:279166/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTTCGCCGGGTCGGaaa > 2:573757/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGgaa > 2:369078/1‑90 (MQ=255) tGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTTCGCCGGATCGGAAAAc > 1:510559/1‑90 (MQ=255) gcggcgGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAAcc < 1:82131/90‑1 (MQ=255) cggcggTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCg < 2:212570/90‑1 (MQ=255) gTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATccc < 2:129074/90‑1 (MQ=255) | GCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCC > NZ_CP009273/115542‑115714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |