| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,503,965 | T→C | D67D (GAT→GAC) | hicB → | type II toxin‑antitoxin system antitoxin HicB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,503,965 | 0 | T | C | 93.8% | 42.2 / ‑3.4 | 16 | D67D (GAT→GAC) | hicB | type II toxin‑antitoxin system antitoxin HicB |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (7/8); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
GCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAG > NZ_CP009273/1503879‑1504049 | gCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCAc < 2:185902/90‑1 (MQ=255) cTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAg < 1:560832/90‑1 (MQ=255) gACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCAc > 1:367542/1‑73 (MQ=255) gACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCAc < 2:367542/73‑1 (MQ=255) tttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc < 1:30448/90‑1 (MQ=255) tttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc < 2:426977/90‑1 (MQ=255) tttGATTTTTATTTTGAAGACAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc < 1:408998/90‑1 (MQ=255) ttATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTaa < 1:176791/90‑1 (MQ=255) ttATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTaa > 1:529348/1‑90 (MQ=255) aaGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGc < 2:674679/90‑1 (MQ=255) ttATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCtt > 1:479111/1‑90 (MQ=255) cTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTAc < 2:546411/90‑1 (MQ=255) ttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAg > 1:429688/1‑90 (MQ=255) gTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGAATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAag > 2:498458/1‑90 (MQ=255) cACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAagag > 1:195664/1‑90 (MQ=255) cACGACCACCTTTTTGCAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAATGGCTTTTTTACAGTCATAAAACACTCAGCAagag > 1:414710/1‑90 (MQ=255) | GCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAG > NZ_CP009273/1503879‑1504049 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |