Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,547,935	A→G	V54A (GTA→GCA)	adhP ←	alcohol dehydrogenase AdhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,547,935	0	A	G	100.0%	75.8 / NA	24	V54A (GTA→GCA)	adhP	alcohol dehydrogenase AdhP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/16);  total (8/16)

ACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTC  >  NZ_CP009273/1547859‑1548002                                                                             |                                                                    aCGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTc                                                        <  1:59716/90‑1 (MQ=255)    aTCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCAcc                                                     <  1:168363/90‑1 (MQ=255)     tCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCa                                                    >  1:635253/1‑90 (MQ=255)             ttttAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGcc                                            <  2:685014/90‑1 (MQ=255)                    gAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTa                                     <  2:453326/90‑1 (MQ=255)                    gAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTa                                     <  2:565010/90‑1 (MQ=255)                      ggTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAAc                                   >  2:21100/1‑90 (MQ=255)                      ggTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAAc                                   >  2:248421/1‑90 (MQ=255)                          acacCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGa                               <  2:711298/90‑1 (MQ=255)                               tGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                          <  1:709412/90‑1 (MQ=255)                                    ccACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTAt                     <  2:277735/90‑1 (MQ=255)                                         tCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACAt                <  1:650795/90‑1 (MQ=255)                                         tCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACAt                <  2:66584/90‑1 (MQ=255)                                         tCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACAt                <  2:635253/90‑1 (MQ=255)                                          cTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGAGCGGTATGACATa               >  1:341740/1‑90 (MQ=255)                                           tGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATac              <  2:259397/90‑1 (MQ=255)                                              caccacACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATAcacc           <  2:145892/90‑1 (MQ=255)                                                 cacaCCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                          <  1:193607/72‑1 (MQ=255)                                                 cacaCCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                          >  2:193607/1‑72 (MQ=255)                                                   cacCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATac              <  2:221622/82‑1 (MQ=255)                                                   cacCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATac              >  1:221622/1‑82 (MQ=255)                                                      cGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACt   >  2:357197/1‑90 (MQ=255)                                                      cGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACt   >  1:78951/1‑90 (MQ=255)                                                       gATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTc  <  2:367837/90‑1 (MQ=255)                                                                             |                                                                    ACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTC  >  NZ_CP009273/1547859‑1548002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑