| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,673,542 | A→G | K287E (AAG→GAG) | ydgH → | DUF1471 family protein YdgH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,673,542 | 0 | A | G | 97.1% | 91.2 / ‑3.4 | 34 | K287E (AAG→GAG) | ydgH | DUF1471 family protein YdgH |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (11/22); total (11/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01 | |||||||||||
ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATC > NZ_CP009273/1673458‑1673617 | atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa < 2:449865/90‑1 (MQ=255) tgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAg < 2:554275/90‑1 (MQ=255) ggTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAg < 2:47049/89‑1 (MQ=255) ggTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAg > 1:47049/1‑89 (MQ=255) tCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTg > 1:96393/1‑90 (MQ=255) cccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGc < 1:215090/90‑1 (MQ=255) cccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGc < 1:87698/90‑1 (MQ=255) cccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGc < 1:46377/90‑1 (MQ=255) ccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt < 2:318575/90‑1 (MQ=255) ccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCt < 1:128722/90‑1 (MQ=255) cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa < 2:331967/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa > 2:76687/1‑82 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa < 1:76687/82‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 1:551894/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 2:77144/90‑1 (MQ=255) aTCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACcac < 1:274271/90‑1 (MQ=255) aTCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACcac < 1:299281/90‑1 (MQ=255) aTTCTCTGGCAACCACGGAAACATGACCAAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAAAAGGGTGCAAAGTTTTACCACATCAc < 1:690412/90‑1 (MQ=255) ctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGcc > 2:560530/1‑90 (MQ=255) gCAACATGACCGAAGTCTCCTATCAGGTTGCAAAACGTGCCGCGGAAAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAAc < 2:57823/90‑1 (MQ=255) aaCATGCCCGAATTCTCCTATCAGGTTGGGAAACGGGCCGGGGAAAAAGGTGCTAATTATTACCACATCCCCCGCCAGTGGCAGGAACGt < 1:600179/90‑1 (MQ=255) aCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTg > 2:114354/1‑90 (MQ=255) aTGACCGAGGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGt > 2:480128/1‑90 (MQ=255) gcccGAATTCTCTTGTCAGGTTGCAAAAGGGGCCGGGGAGAAAGGTTTAAAGTTTTCCCACATCCCCCGCCAGTGGCAGGAACGTGGtaa < 1:368020/88‑1 (MQ=255) aCCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa < 1:46266/42‑1 (MQ=255) aCCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa > 2:46266/1‑42 (MQ=255) ccTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCg > 2:485420/1‑90 (MQ=255) ggTTGCAAAACGTGCCGAGGAAAAAGGTGCAAATTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc < 1:709069/90‑1 (MQ=255) tGCGAAACGTGCCGGGGAGAAAGGTGCTAAGTATTACCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGa < 2:25313/90‑1 (MQ=255) tGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGa > 2:199762/1‑90 (MQ=255) tGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGGGGGAATAACCTGACCGTCAGCGCAGa > 1:325610/1‑90 (MQ=255) tGCAAAAGGGGCCGGGGAAAAGGGTGATAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGa < 1:560530/90‑1 (MQ=255) gCGAAACGTGCCGCGGAGAAAGGTGCTAAGTGTTACCACATCACCCGCCAGTGGCAGGAACGTGGGAAAAACCTGACCGTCAGCGCCGAt > 1:634767/1‑90 (MQ=255) caaaaCGTGCCGGGGAAAAAGGTGCTAATTATTACCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc < 2:604198/88‑1 (MQ=255) | ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATC > NZ_CP009273/1673458‑1673617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |