Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,753	(A)6→5	coding (377/435 nt)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,748	0	A	.	100.0%	23.2 / NA	7	coding (372/435 nt)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (4/3);  total (4/3)

AACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGA  >  NZ_CP009273/1613672‑1613828                                                                             |                                                                                 atcTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAAC‑AAAAACCTGAcggc                                                                    >  1:238786/3‑90 (MQ=255) aaCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAAC‑AAAAACCTGAcggc                                                                    >  2:138854/1‑90 (MQ=255)                     gcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAAC‑AAAAACCTGACGGCGGAAGAAGTGGCAACACTTg                                                <  1:138854/90‑1 (MQ=255)                                          cAATTAGTTGGCCAGGACCTGCCCCAAAAATTAAC‑AAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTcc                           <  2:213766/90‑1 (MQ=255)                                                  tGGCCAGGACCTGCACCAAGAATTAAC‑AAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTaa                   <  1:55801/90‑1 (MQ=255)                                                        ggACCTGCACCAAGAATTAAC‑AAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACaaaa             >  2:1016/1‑90 (MQ=255)                                                                   aaGAATTAAC‑AAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGa  >  2:266158/1‑90 (MQ=255)                                                                             |                                                                                 AACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGA  >  NZ_CP009273/1613672‑1613828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑