| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 700,026 | 0 | T | G | 56.2% | 12.3 / 14.8 | 16 | G209G (GGT→GGG) | nagE | PTS N‑acetyl glucosamine transporter subunit IIABC |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (9/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCAACCGTCTGCTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGA > NZ_CP009273/699937‑700105 | atcaACCGTCTGCTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGt > 1:191346/1‑90 (MQ=255) ctgctgATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGgtttt > 1:417244/1‑89 (MQ=255) ctgctgATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGATTTc > 2:336722/1‑90 (MQ=255) gATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACgg < 2:68541/90‑1 (MQ=255) ggTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTCCGCGGTGACATTaaa > 1:452049/1‑89 (MQ=39) gTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAAcc < 1:107330/90‑1 (MQ=255) gTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAAcc < 2:44980/90‑1 (MQ=255) ggTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGGTTTCCACGGTGACATTAACCGCTTCTATGc > 1:205970/1‑90 (MQ=255) gAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGTTTTTCACGGGGACATTAACCGCTTCTATTCCGGGGa > 1:241033/1‑90 (MQ=255) ccATCGCCTGGTCCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGTTTTCCACGGGGACATTAACCGCTTCTATGCCGGTGGCGGCa > 1:611/1‑90 (MQ=255) tCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTTCACGGGGGCATTAACCGGGTCTATGCCCGGGGCGGCCCcg > 2:262896/1‑90 (MQ=255) gCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTCCACGGGGGCATTAACCGCTTCTATGCCGGTGACGGCACcgcg > 1:162657/1‑90 (MQ=255) tGGTGAATTCACCAACGCGGCGGGGACGGTTTTTCACGGGGGCATTAACCGCTTCTATGCCGGGGGCGGCCCCGCGGGGGTGTTTATGTc > 1:283952/1‑90 (MQ=255) ggTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTcc < 1:259351/90‑1 (MQ=255) ggTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTcc < 1:62488/90‑1 (MQ=255) ggTGAATTCACCAACGCGGCGGGGACGGGTTTCCACGGGGACATTAACCGCTTCTATTCCGGGGGGGGGACCGCGGGGGTGGTTATGTcc > 2:375544/1‑90 (MQ=255) aCGCGGCGGGGACGGGTTTCCACGGTGACATTAACCGCTTCTATGCCGGGGACGGCCCCGCGGGGGTGTTCATGTCCGGCTTCTTccccc > 2:320196/1‑88 (MQ=255) | ATCAACCGTCTGCTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGA > NZ_CP009273/699937‑700105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |