| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,571,380 | 0 | A | C | 64.3% | 2.3 / 11.3 | 14 | V166G (GTA→GGA) | yddB | TonB‑dependent receptor plug domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/9); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCAAGATAA > NZ_CP009273/1571296‑1571463 | gTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCg > 2:403777/1‑90 (MQ=255) aaCCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCa > 1:22218/1‑90 (MQ=255) aCGCGTAGTGCGATACCCCAATTTCCCCTTGCTATCATCAGCGTTAAAGCGTTTGATCTTTGCATCAATTCCCCCGCCATTGAAGCGAcc < 1:437127/90‑1 (MQ=255) aCGCGTAGTGCGATACCCAAATTTCCCCTTGCTATCACCAGCGTTGAAGCGTTTGATCTTTGCATCAATTCCCCCGCCATTGAAGCGAcc < 2:45829/90‑1 (MQ=255) gTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAAt > 2:218666/1‑90 (MQ=255) aTTTCCCCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAAc < 1:361418/90‑1 (MQ=255) caGGGTTGAAGTGTTTGATCTTTCCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACAt < 2:458462/90‑1 (MQ=255) gggTTAAAGCGTTTGATCTTTCCATAAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTg < 2:458928/88‑1 (MQ=255) ttttCCATCATTTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATc < 2:51497/89‑1 (MQ=255) cTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATc > 2:37105/1‑90 (MQ=255) cTTCGCATCATGTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATc < 2:316204/90‑1 (MQ=255) tttGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCa < 1:406828/90‑1 (MQ=255) ttGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCaa < 1:199132/90‑1 (MQ=255) tCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGAAAGCTGACATCAAGataa > 1:342036/1‑90 (MQ=255) | GTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCAAGATAA > NZ_CP009273/1571296‑1571463 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |