| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,001,832 | 0 | T | G | 53.8% | 11.6 / 14.9 | 13 | G25G (GGT→GGG) | yedE | selenium metabolism membrane protein YedE/FdhT |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (7/0); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCT > NZ_CP009273/2001744‑2001915 | cAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTa < 1:369199/90‑1 (MQ=255)atgagGCTAAATTCATGTCATGGCAGCAATTCAAACTCGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTa < 2:265356/88‑1 (MQ=255) tACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATtctctc < 1:245122/90‑1 (MQ=255) atgtcatgGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTcttctttc > 1:357437/1‑88 (MQ=255) atgtcatgGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCtactttc > 1:402378/1‑88 (MQ=255) atgGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTCTCTACTTACTAttt > 1:192069/1‑90 (MQ=255) aaCACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCa < 2:3859/90‑1 (MQ=255) gCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTCTCTACTTACTATTTTTGCATTACTGGCACCttt > 1:361673/1‑90 (MQ=255) gggCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGGGAAt > 1:317894/1‑90 (MQ=255) cATCGCGGCGGGGATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGGGAATTTACCCGTTGGGGCGGCCa > 1:130108/1‑90 (MQ=255) cATCGCGGCGGGGATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGGGAATTTACCCGTTGGGGCGGCCa > 1:260345/1‑90 (MQ=255) cgcgGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCt < 1:317599/90‑1 (MQ=255) ggcggGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCt < 2:294212/90‑1 (MQ=255) | CAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCT > NZ_CP009273/2001744‑2001915 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |