| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,556,746 | 0 | A | G | 64.3% | 3.9 / 10.6 | 14 | intergenic (+270/‑205) | BW25113_RS12760/BW25113_RS12765 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (3/2); new base G (4/5); total (7/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACG > NZ_CP009273/2556668‑2556835 | aCCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCaa > 1:146569/1‑90 (MQ=255) cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCGACTCCCACCaaa > 2:66056/1‑90 (MQ=255) cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCaaa > 2:82372/1‑90 (MQ=255) aTCTTTCCGTTTTCAGGTGGGATACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCGACTCCCACCAAAGcc < 2:239730/90‑1 (MQ=255) cGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCGACTCCCACCAAAGCCAACAAGa > 2:250484/1‑90 (MQ=255) cAGGT‑GGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCGACTCCCACCAAAGCCAACAAGATGGGAgg < 1:207710/90‑1 (MQ=255) ggCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCGACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGc > 2:91803/1‑90 (MQ=255) ccgACGTTGCACAATTGATTAATTTCTGAACATTCAAATCGACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATc < 1:5456/90‑1 (MQ=255) ttGCACAATTGATTAATTTCTGAACATTCAAATCAACTc < 1:3661/39‑1 (MQ=255) ttGCACAATTGATTAATTTCTGAACATTCAAATCAACTc > 2:3661/1‑39 (MQ=255) tGATTAATTTCTGAACATTCAAATCGACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAg < 1:250484/90‑1 (MQ=255) ttCAAATCGACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtc > 1:434860/1‑90 (MQ=255) ttCAAATCGACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtc < 1:66056/90‑1 (MQ=255) aaTCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTCTAATtcaatc < 1:426904/90‑1 (MQ=255) aaCTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACg > 2:242974/1‑90 (MQ=255) | ACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACG > NZ_CP009273/2556668‑2556835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |