| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,126 | 0 | T | C | 61.5% | 2.9 / 12.0 | 13 | I425T (ATC→ACC) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (5/0); new base C (0/8); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTA > NZ_CP009273/3214050‑3214214 | aTCGGTTATAATTTTGCCAGGGAGTTTTCCCGCCAGCGCGTACGGGTGGCCGGAACGCTAAATAACCCAAAAA‑CGACCCGCATTGAACCg < 2:94251/90‑1 (MQ=255) ataACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCcactga > 2:384098/1‑90 (MQ=255) cggggagaTTTCCCGCCAGCGGGTAGGGGGGGCGGAAACCCT‑AAAAACCCCAAAACCGACCCGAATTAACCCGCCACTGACACTGACCAt < 2:106326/86‑1 (MQ=255) gagaTTTCCCCCCGGCGCGT‑CTGGGGGCCCGAACCCCTAAAAACCCCAAAACCGACCCGCATTAACCCGCCACTGACACTGACCATTGaa < 2:105734/90‑1 (MQ=255) gagaTGTCCCGCCAGCGCGTACGGGTGGCGGAACCGCTAAAAAACCCAAAAA‑CGACCCGCATTAAACCGCCACTGACACTGACCATTGaa < 1:153726/90‑1 (MQ=255) ggaTGTCCCCCGAGCGCTTGCGGGGGCCCGGAACGCTCAAAACCGCAAAAA‑CGCCCCGTATGGAACCGCCACTGACACTGACCATTGAAc < 2:381141/89‑1 (MQ=255) agttGTCCCGCCGGCGCGTCCGGGGGCCCGGACCGCTAAAAACCGCAAAAA‑CGACCCGCATTAAACCGCCACTGACACTGACCATTGAAc < 1:83234/87‑1 (MQ=255) agaTTTCCCGCCGGCGGGTTTGGGGGGCGGGACCGCTAAAAAACCCAAAAA‑CGACCCGCATTAAACCGCCACTGACACTGACCATTGAAc < 1:300927/90‑1 (MQ=255) tCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGtgtg > 1:382479/1‑90 (MQ=255) gCCGGCGCGTACGGGGGGCCGAACCGCTAAAAACCCCAAAAA‑CGACCCGAATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAAc < 1:449929/90‑1 (MQ=255) gAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTa > 1:45370/1‑90 (MQ=255) cGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAgg > 1:199589/1‑90 (MQ=255) aTCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTa > 2:302869/1‑90 (MQ=255) | ATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTA > NZ_CP009273/3214050‑3214214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |