| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,273,830 | 0 | A | C | 64.3% | 3.9 / 10.0 | 14 | T85T (ACA→ACC) | agaV | PTS N‑acetylgalactosamine transporter subunit IIB |
| Reads supporting (aligned to +/- strand): ref base A (3/2); new base C (0/9); total (3/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAAT‑CCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACTACGCCAATGGCA > NZ_CP009273/3273750‑3273912 | cGTTTCTGGCCGCTGAAAAAAGTTATCGACAACATTCACCGCCCCCCCGATCGACAAAAAAT‑CCTGCTGGTTTGTAAAACACCCGCCGAt < 1:100134/90‑1 (MQ=255) ggACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAAT‑CCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGa > 2:260945/1‑90 (MQ=255) cTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAAT‑CCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTg > 2:95395/1‑90 (MQ=255) ttATCGACAACATTCATCGCGCCGCCGATCGACAGAAAAT‑CCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTg > 2:106185/1‑90 (MQ=255) caccgcgCCGCCGATCGACAAAAAAT‑CCTGCTGGTTTGAAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAAt < 1:377011/87‑1 (MQ=255) ccgccgATGGACAGAAAAT‑CCTGGTGGTTTGAAAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTa < 2:279227/90‑1 (MQ=255) ttgACAAAAAAT‑CCTGCTGGTTTGTAAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTgg < 1:140151/88‑1 (MQ=255) tCGACAGAAAAT‑CCTGCTGGTTTGTAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTgg < 1:180918/90‑1 (MQ=255) tCGAAAAAAAAC‑CCTGTTGGTTTGAAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTgg < 2:95480/90‑1 (MQ=255) aaaaaaT‑CCTGCTGGTTTGTAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAata < 1:166566/88‑1 (MQ=255) aaaatT‑CTTGCTGTTTGTTAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAatat < 1:456061/89‑1 (MQ=255) aaaaaT‑CCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAatat < 1:106185/89‑1 (MQ=255) aaaaTCCCTGTTGGTTTGAAAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAatat < 1:465218/90‑1 (MQ=255) gaaaaaCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACTACGCCAATGGCa < 1:218876/88‑1 (MQ=255) | CGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAAT‑CCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACTACGCCAATGGCA > NZ_CP009273/3273750‑3273912 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |