| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,659,058 | 0 | T | C | 61.1% | ‑0.5 / 10.1 | 18 | D38G (GAT→GGT) | gadX | acid resistance transcriptional activator GadX |
| Reads supporting (aligned to +/- strand): ref base T (4/3); new base C (0/11); total (4/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGC > NZ_CP009273/3658977‑3659140 | aTCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaaa > 1:48408/1‑90 (MQ=255) aaatGAAAGCGTGCCCCTTGACACAAATAAAAAATTTTCAACACACTTATCTACTCAAATTTGGCTTGCACCCGAAAAAACCAGGTCAcc < 1:96927/89‑1 (MQ=255) ttccccTTAACAAAAATAAAAATTTTCCAACCCCCTTATCTTCTAAATTTTGGCTTCCACCCGAAAAACCCAGGCCACCGCCATTAAAAt < 2:352719/87‑1 (MQ=255) cccTTGACACAAATAAAAAATTTCCAACACACTTATCTCCTCGATTTTGGCTTGCATCCGCAAAACCCAGGTCACCGCCATTAAAATAGc < 1:18578/90‑1 (MQ=255) cccTTAACACAAATAAAAAATTTCCAACACATTTATCTCCTCGATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGc < 2:39772/90‑1 (MQ=255) cacGAATACAAAATTTTCAACCCATTTATCTCCTCAATTTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTc < 1:63664/90‑1 (MQ=255) gAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCAcc > 1:10101/1‑90 (MQ=255) aTACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGa > 2:11100/1‑80 (MQ=255) aTACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGa < 1:11100/80‑1 (MQ=255) taaaCACTTTTATTTACTAAATTTTGGCTTCCACCCGAAAAACCCGGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg < 2:48408/88‑1 (MQ=255) tCAACACCTTTATCTACTCAAATTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg < 1:131979/90‑1 (MQ=255) cccTTATCTACTAGATTTTGTTTTCCACCCAAAAAAACCGGCTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAata < 2:233428/88‑1 (MQ=255) cGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCtt > 1:244477/1‑90 (MQ=255) tGGTTTGCACCCAAAAAAACCGGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc < 2:214675/90‑1 (MQ=255) tGGCTTCCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc < 1:257262/90‑1 (MQ=255) tGGCTTCCACCCAAAAAAACCAGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc < 2:44965/90‑1 (MQ=255) tGGCTTCCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc < 1:25407/90‑1 (MQ=255) gCTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGc < 1:434377/90‑1 (MQ=255) | ATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGC > NZ_CP009273/3658977‑3659140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |