Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,896,6280AC54.5% 19.7 / 28.9 22V145G (GTC→GGC) bglB6‑phospho‑beta‑glucosidase BglB
Reads supporting (aligned to +/- strand):  ref base A (8/2);  new base C (0/12);  total (8/14)
Fisher's exact test for biased strand distribution p-value = 1.41e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-05
Rejected as consensus: Frequency below/above cutoff threshold.

GATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCGGCTTGAT  >  NZ_CP009273/3896543‑3896710
                                                                                     |                                                                                  
gATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGc                                                                                <  2:189009/90‑1 (MQ=255)
       ttAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTa                                                                         <  2:58938/90‑1 (MQ=255)
               cAGCCATAACGCCATTTTTTGTTGGTAGCGAGTAAAGCCCGTGGGGGCGTAATGCTCGAAGTGGTCGATGCCCGCTCGATTACCCCAAcc                                                                 <  1:394338/90‑1 (MQ=255)
                              ttttTGTTGGGGGCGAGAAAAGCCCGGGGGGGGCTAACGATCGAAGTAATCGATTCCCGCTAAATTACCCAACCCGCCGTAGTTTTTCAc                                                  <  2:318649/90‑1 (MQ=255)
                              ttttTGTGGGTAGGGAGAAAAGCCCGGGGGGGCGTAATGCTCGAAGGGGCCGATGCCCGCTCGATTACCCCACCCGCCGTAGTTTTTCAc                                                  <  2:323065/90‑1 (MQ=255)
                              tttATTTGGGTGGGGAGAAAAGCCCGGGGGGGCGTAATGCTGGAAGTGATCGATGCCCGCTCGATTACCCCAACCGCCGTAGTTTTTCAc                                                  <  2:134900/90‑1 (MQ=255)
                                   gTTGGTAGGGAAAAAAGCCCGGGGGGGCGTAATGCTGGAAGTGGTCGATCCCCGCTCGATTACCCCACCCGCCGTAGTTTTTCACCAGcc                                             <  2:285115/90‑1 (MQ=255)
                                   gTTGGTAGCGAGAAAAGCCCGGGGGGGGGTAATGCTGGAAGTGATCGATGCCCGCTCGATTACCCCAACCGCCGTAGTTTTTCACCAGcc                                             <  2:269866/90‑1 (MQ=255)
                                   gTTGGTAGCGAGAAAAGCCCGGGGGGGCGTAATGCTCGAGGGGGTCGATGCCCGCTCGATTACCCAAACCGCCGTAGTTTTTCACCAGcc                                             <  2:437893/90‑1 (MQ=255)
                                       gTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCata                                         >  1:358675/1‑90 (MQ=255)
                                                 aaGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCATCAGCCCATATGGCATTTCg                               >  2:380162/1‑90 (MQ=255)
                                                       gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAGTgg                         >  1:89204/1‑90 (MQ=255)
                                                       gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                         >  2:226916/1‑90 (MQ=255)
                                                       gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                         >  2:424826/1‑90 (MQ=255)
                                                         gggggCGTAATGCTGGAGGGGGTCGATGCCCGCTCGTTTACCCCACCCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGa                       <  2:149330/88‑1 (MQ=255)
                                                         gCGGGCGTAATGCTCGAGGTGGTCGATGCCCGCTCGATTACCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGa                       <  1:142547/90‑1 (MQ=255)
                                                          ggggCATAATGCTCGAGGGGGTCGATCCCCCCTCGATTACCCCACCCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGAt                      <  2:444577/89‑1 (MQ=255)
                                                               gTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGt                 >  1:289170/1‑90 (MQ=255)
                                                                      tCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGc          >  1:180930/1‑90 (MQ=255)
                                                                       ggaggTGGTCGATGCCCGCTATTTTACCCCACCCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCg         <  2:259379/86‑1 (MQ=255)
                                                                           ggggtCGATGCCCGCTCTATTACCCCACCCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCGGCtt     <  2:423998/86‑1 (MQ=255)
                                                                              gtCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCGGCTTGAt  >  2:78853/2‑90 (MQ=255)
                                                                                     |                                                                                  
GATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCGGCTTGAT  >  NZ_CP009273/3896543‑3896710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑