| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,956 | 0 | C | A | 53.8% | 1.7 / 10.8 | 13 | L57L (CTG→CTT) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base A (0/7); total (3/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058870‑4059038 | aaTTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGa < 1:387014/90‑1 (MQ=255) gggagtagagaAAAAACCCGGTGAGAATATCGGTAACCGCGAAAAAAACCTCCGCCACAAGAAAGATAATCCCGCCATAGTAAGCAGGCa < 1:294096/88‑1 (MQ=255) aaaCCCGGTGAGCATATGGGAAAACGGGGAAAAAAACTTCCCCACAAAAAAGAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 1:105009/90‑1 (MQ=255) aaaCCCGGTGAGCATAGCGGAAACCGCGGAAAAAACCTCCGCCACCAGAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 1:287359/90‑1 (MQ=255) ccGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGta > 2:413553/1‑90 (MQ=255) ggggAGCATATGGGAAAACGGGGAAAAAACCTCCCCCCCAAGAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata < 1:222480/87‑1 (MQ=255) ggTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata > 1:234285/1‑90 (MQ=255) tttgggTAACCGCGAAAAAAACCCCCCCCACAAAAAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTga < 2:297484/86‑1 (MQ=255) tatCGGAAAACGGGAAAAAAAACTTCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTga < 2:81725/90‑1 (MQ=255) tCGGAAAACGCGGAAAAAAACTTCCCCCCCAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg < 1:202506/90‑1 (MQ=255) tCGGAAAACGCGGAAAAAAACTCCCCCACAAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg < 1:57801/90‑1 (MQ=255) ccGGAAAACGGGAAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg < 2:7049/89‑1 (MQ=255) tCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCaga > 1:270204/1‑90 (MQ=255) | AATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058870‑4059038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |