| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,440,600 | 0 | T | G | 61.1% | 1.5 / 14.5 | 18 | G274G (GGT→GGG) | ytfQ | galactofuranose ABC transporter substrate‑binding protein YtfQ |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (11/0); total (14/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.08e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCCATTGACGGTGTACCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACC > NZ_CP009273/4440513‑4440682 | ttCCATTGACGGTGTACCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGcc > 1:247174/1‑90 (MQ=255) tCCATTGACGGTGTACCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTccc < 1:19718/90‑1 (MQ=255) tCCATTGACGGTGTACCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGccc > 1:113405/1‑90 (MQ=255) ggACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCGCCTTCGACGCGCTggg > 1:92314/1‑89 (MQ=255) aCATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgaga < 2:128438/90‑1 (MQ=255) cATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCGCCTTCGACGCGCTGGAGaa > 1:30038/1‑90 (MQ=255) aaGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCCCCCTCCACGCGCTGGGGaaaaaaaaaa > 2:207476/1‑90 (MQ=255) gCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCGCCTTCGACGCGCTGGGGGAAAACaaaaaa > 1:431065/1‑90 (MQ=255) gatgatGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCCCCCTCCACGCGCTGGGGaaaaaaaaaaaaaa > 2:237917/1‑88 (MQ=255) atgatgGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCCCCCTCCACGCGCTGGGGAAAAAAAAAAAAAAc > 1:358074/1‑90 (MQ=255) gatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCAcc < 1:244174/90‑1 (MQ=255) gaagcgaaTGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCCCCCTCCACCCGCCGGGGGAAAAAAAAAAAAAAGGCACCCTGccc > 2:302582/1‑89 (MQ=255) gaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGGGCCCCCCTCGACGCGCTGGGGAAAAAAAAAAAAAAAGGCACCAAGccc > 2:303405/1‑89 (MQ=255) gAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAAcc > 1:189926/1‑90 (MQ=255) gACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAAACGGCACCATGCCTGAAAAGCTGACGTTAACCaaaac > 1:285864/1‑88 (MQ=255) gACGCCGAATATGGCAGGGGCCGCCCTCCACGCGCGGGGGAAAAACAAAAAAAAAGGCCCCCACCCCGGAAAGCCGACGTTAACCaaaac > 2:303489/1‑88 (MQ=255) cGCCGAATATGGCAGGTCCCCCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAACTGACGTTAACCAAAACCa > 1:353257/1‑90 (MQ=255) tGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTAcc < 2:395599/90‑1 (MQ=255) | TTCCATTGACGGTGTACCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACC > NZ_CP009273/4440513‑4440682 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |