Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,004,531	A→C	T151P (ACG→CCG)	yedL →	GNAT family N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,004,531	0	A	C	100.0%	30.1 / NA	11	T151P (ACG→CCG)	yedL	GNAT family N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (3/8);  total (3/8)

ATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACC  >  NZ_CP009273/2004445‑2004612                                                                                       |                                                                                  aTCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGCCGc                                                                                <  2:192798/90‑1 (MQ=255)         tATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGCCGCTATGCGaa                                                                        <  2:350060/90‑1 (MQ=255)                aGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGCCGCTATGCGAAAATAATg                                                                 <  1:231355/90‑1 (MQ=255)                        ttCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGCCGCTATGCGAAAATAATGAATTGCCa                                                         <  2:461962/90‑1 (MQ=255)                               aTTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGCCGCTATGCGAAAATAATGAATTGCCATGAGCCa                                                  <  2:272277/90‑1 (MQ=255)                                        cGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGCCGCTATGCGAAAATAATGAATTGCCATGAGCCAGACgcagca                                         >  1:178985/1‑90 (MQ=255)                                                       gACTTGATCCACACAGTCGATTTTTATCATTGCCGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGa                          <  2:178985/90‑1 (MQ=255)                                                                  cacaGTCGATTTTTATCATTGCCGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGcc               >  2:350967/1‑90 (MQ=255)                                                                           tttttATCATTGCCGCTATGCGAAAATAATGAATTGCCATGAGCCAGACgcagc                                          >  1:92758/1‑54 (MQ=255)                                                                           tttttATCATTGCCGCTATGCGAAAATAATGAATTGCCATGAGCCAGACgcagc                                          <  2:92758/54‑1 (MQ=255)                                                                               tATCATTGCCGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCAcc  <  1:220801/90‑1 (MQ=255)                                                                                       |                                                                                  ATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACC  >  NZ_CP009273/2004445‑2004612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑