Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,266,497	A→G	K219E (AAA→GAA)	yejA →	extracellular solute‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,266,497	0	A	G	100.0%	30.7 / NA	11	K219E (AAA→GAA)	yejA	extracellular solute‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/6);  total (5/6)

GAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCT  >  NZ_CP009273/2266424‑2266572                                                                          |                                                                            gAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGGAAATGGGGCAAAatat                                                             <  1:42270/90‑1 (MQ=255) gAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGGAAATGGGGCAAAatat                                                             <  2:279154/90‑1 (MQ=255) gAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTACTGGGAAATGGGGCAAAatat                                                             <  2:278626/90‑1 (MQ=255)                 aGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGGAAATGGGGCAAAATATTGTCTATTCCCGtgtg                                             >  1:370245/1‑90 (MQ=255)                  gCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGGAAATGGGGCAAAATATTGTCTATTCCCGTGTGa                                            <  1:297839/90‑1 (MQ=255)                                      ccGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGGAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAAc                        >  1:118859/1‑90 (MQ=255)                                      ccGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGGAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAAc                        >  1:311633/1‑90 (MQ=255)                                        gCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGGAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACtt                      <  1:109301/90‑1 (MQ=255)                                               aGTGGTCCGTACCGCGTTACGTCCTGGGAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTa               <  2:43756/90‑1 (MQ=255)                                                            gcgTTACGTCCTGGGAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCt  >  2:175430/1‑90 (MQ=255)                                                            gcgTTACGTCCTGGGAAATGGGGCAAAATACTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCt  >  1:217381/1‑90 (MQ=255)                                                                          |                                                                            GAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCT  >  NZ_CP009273/2266424‑2266572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑