Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,511,652	A→G	noncoding (59/76 nt)	BW25113_RS12505 ←	tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,511,652	0	A	G	100.0%	43.9 / NA	15	noncoding (59/76 nt)	BW25113_RS12505	tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/7);  total (8/7)

AAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTC  >  NZ_CP009273/2511567‑2511713                                                                                      |                                                              aaGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGGTCGa                                                           <  2:424171/90‑1 (MQ=255)       tctcCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCt                                                     >  2:153225/1‑90 (MQ=255)       tctcCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCt                                                     >  2:331339/1‑90 (MQ=255)                        aGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGcatgccat                                    >  2:422680/1‑90 (MQ=255)                             cACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAg                               >  2:90030/1‑90 (MQ=255)                                   gCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGctct                         >  1:454293/1‑90 (MQ=255)                                           gTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTg                 >  1:110240/1‑90 (MQ=255)                                           gTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTg                 <  1:64082/90‑1 (MQ=255)                                            tACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGa                <  2:205609/90‑1 (MQ=255)                                            tACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGa                <  2:424730/90‑1 (MQ=255)                                                aaGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCta            >  1:305151/1‑90 (MQ=255)                                                      gCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGa                >  1:176424/1‑80 (MQ=255)                                                      gCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGa                <  2:176424/80‑1 (MQ=255)                                                         gggTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGt   <  2:406425/90‑1 (MQ=255)                                                          ggTGCAAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTc  <  2:454293/90‑1 (MQ=255)                                                                                      |                                                              AAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTC  >  NZ_CP009273/2511567‑2511713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑