Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,599,995	A→C	K125N (AAA→AAC)	hyfE →	hydrogenase 4 membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,599,995	0	A	C	100.0%	21.7 / NA	9	K125N (AAA→AAC)	hyfE	hydrogenase 4 membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (3/6);  total (3/6)

GATGGCACTGCTCGCGGCGTTAATTGTCCTGCTTTGCGCATTTGTCGTTCAGCCCGTGAAGCTACCGATGGCTACCGGGCTGAAACCGGCGCTGGCGGTAGCGTTAGGTCATTTTCTGCTTGGCCTGCTGTGCATTGTCAGCCAGCGCAATATCCTGCGGCAAATTTT  >  NZ_CP009273/2599911‑2600078                                                                                     |                                                                                    gatgGCACTGCTCGCGGCGTTAATTGTCCTGCTTTGCGCATTTGTCGTTCAGCCCGTGAAGCTACCGATGGCTACCGGGCTGAACCCGgc                                                                                >  1:275146/1‑90 (MQ=255)          gCTCGCGGCGTTAATTGTCCTGCTTTGCGCATTTGTCGTTCAGCCCGTGAAGCTACCGATGGCTACCGGGCTGAACCCGGCGCTGGCGGt                                                                       <  1:151587/90‑1 (MQ=255)                 gcgTTAATTGTCCTGCTTTGCGCATTTGTCGTTCAGCCCGTGAAGCTACCGATGGCTACCGGGCTGAACCCGGCGCTGGCGGTAGCGTTa                                                                <  1:469734/90‑1 (MQ=255)                                            gTCGTTCAGCCCGTGAAGCTACCGATGGCTACCGGGCTGAACCCGGCGCTGGCGGTAGCGTTAGGTCATTTTCTGCTTGGCCTGCTGTGc                                     <  1:443016/90‑1 (MQ=255)                                                      ccGTGAAGCTACCGATGGCTACCGGGCTGAACCCGGCGCTGGCGGTAGCGTTAGGTCATTTTCTGCTTGGCCTGCTGTGCATTGTcagcc                           <  1:348627/90‑1 (MQ=255)                                                        gTGAAGCTACCGATGGCTACCGGGCTGAACCCGGCGCTGGCGGTAGCGTTAGGTCATTTTCTGCTTGGCCTGCTGTGCATTGTcagccag                         <  1:157332/90‑1 (MQ=255)                                                         tGAAACTACCGATGGCTACCGGGCTGAACCCGGCGCTGGCGGTAGCGTTAGGTCATTTTCTGCTTGGCCTGCTGTGCATTGTcagccagc                        >  2:60677/1‑90 (MQ=255)                                                              cTACCGATGGCTACCGGGCTGAACCCGGCGCTGGCGGTAGCGTTAGGTCATTTTCTGCTTGGCCTGCTGTGCATTGTCAGCCAGCGCAat                   >  1:425455/1‑90 (MQ=255)                                                                               gCTGAACCCGGCGCTGGCGGTAGCGTTAGGTCATTTTCTGCTTGGCCTGCTGTGCATTGTCAGCCAGCGCAATATCCTGCGGCAAAtttt  <  1:317228/90‑1 (MQ=255)                                                                                     |                                                                                    GATGGCACTGCTCGCGGCGTTAATTGTCCTGCTTTGCGCATTTGTCGTTCAGCCCGTGAAGCTACCGATGGCTACCGGGCTGAAACCGGCGCTGGCGGTAGCGTTAGGTCATTTTCTGCTTGGCCTGCTGTGCATTGTCAGCCAGCGCAATATCCTGCGGCAAATTTT  >  NZ_CP009273/2599911‑2600078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑