| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,706,273 | T→C | S7P (TCC→CCC) | srmB → | ATP‑dependent RNA helicase SrmB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,706,273 | 0 | T | C | 95.0% | 56.4 / ‑6.2 | 20 | S7P (TCC→CCC) | srmB | ATP‑dependent RNA helicase SrmB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (13/6); minor base A (0/1); total (13/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACAAACAGATGAAGATTGCAGCCGTAACGTCTATAATCAGCGCCCCACACAGAGGTAGAACATGACTGTAACGACTTTTTCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGCCCTCCAGGATAAAGGTTTCACTCGCCCGACCGCCATTCAGGCTGC > NZ_CP009273/2706193‑2706352 | tACAAACAGATGAAGATTGCAGCCGTAACGTCTATAATCAGCGCCCCACACAGAGGTAGAACATGACTGTAACGACTTTTCCCGAACTTg > 1:276484/1‑90 (MQ=255) aaCAGATGAAGATTGCAGCCGTAACGTCTATAATCAGCGCCCCACACAGAGGTAGAACATGACTGTAACGACTTTTCCCGAACTTGAACt > 2:64077/1‑90 (MQ=255) aGATGAAGATTGCAGCCGTAACGTCTATAATCAGCGCCCCACACAGAGGTAGAACATGACTGTAACGACTTTTCCCGAACTTGAACTcga > 2:229375/1‑90 (MQ=255) aTTGCAGCCGTAACGTCTATAATCAGCGCCCCACACAGAGGTAGAACATGACTGTAACGACTTTTCCCGAACTTGAACTCGACGAAAGcc < 2:276484/90‑1 (MQ=255) ttGCAGCCGTAACGTCTATAATCAGCGCCCCACACAGAGGTAGAACATGACTGTAACGACTTTTCCCGAACTTGAACTCGACGAAAGCct > 2:121575/1‑90 (MQ=255) tAACGTCTATAATCAGCGCCCCACACAGAGGTAGAACATGACTGTAACGACTTTTCCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGc < 2:431960/90‑1 (MQ=255) tAACGTCTATAATCAGCGCCCCACACAGAGGTAGAACATGACTGTAACGACTTTTCCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGc < 2:368525/90‑1 (MQ=255) tAACGTCTATAATCAGCGCCCCAAACAGAGGTAGAACATGACTGTACCGACTTTTCCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGc < 2:341910/90‑1 (MQ=255) aCGTCTATAATCAGCGCCCCACACAGAGGTAGAACATGACTGTAACGACTTTTCCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGccc < 1:266401/90‑1 (MQ=255) tCTATAATCAGCGCCCCACACAAAGGTACACCATGCCGGCACCGGCTTTTACCGAAATTGAACCCGACGAAAGCCTGCTGGACGCCCTcc < 2:218495/90‑1 (MQ=255) cAGCGCCCCACACAGAGGTAGAACATGACTGTAACGACTTTTCCCGAACTTGAACTTGACGAAAGCCTGCTGGAAGCCCTCCAGGATaaa > 2:113076/1‑90 (MQ=255) cAGCGCCCCACACAGAGGTAGAACATGACTGTAACGACTTTTCCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGCCCTCCAGGATaaa > 1:176218/1‑90 (MQ=255) aGCGCCCCACACAGAGGTAGAACATGACTGTAACGACTTTTCCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGCCCTCCAGGATAAAg > 2:145289/1‑90 (MQ=255) tAGAACATGACTGTAACGACTTTTCCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGCCCTCCAGGATAAAGGTTTCACTCGCCCGAcc < 1:139359/90‑1 (MQ=255) aGAACATGACTGTAACGACTTTTCCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGCCCTCCAGGATAAAGGTTTCACTCGCCCGACCg > 2:53309/1‑90 (MQ=255) gACTGTAACGACTTTTCCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGCCCTCCAGGATAAAGGTTTCACTCGCCCGACCGCCATTCa > 2:324001/1‑90 (MQ=255) gACTGTAACGACTTTTCCCGAACTTGAACTCGACGAAAGCCAGCTGGAAGCCCTCCAGGATAAAGGTTTCACTCGCCCGACCGCCATTCa > 2:367002/1‑90 (MQ=255) gTAACGACTTTTCCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGCCCTCCAGGATAAAGGTTTCACTCGCCCGACCGCCATTCAGGCt > 1:11842/1‑90 (MQ=255) gTAACGACTTTTCCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGCCCTCCAGGATAAAGGTTTCACTCGCCCGACCGCCATTCAGGCt > 2:48044/1‑90 (MQ=255) aaCGACTTTTCCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGCCCTCCAGGATAAAGGTTTCACTCGCCCGACCGCCATTCAGGCTgc > 1:391895/1‑90 (MQ=255) | TACAAACAGATGAAGATTGCAGCCGTAACGTCTATAATCAGCGCCCCACACAGAGGTAGAACATGACTGTAACGACTTTTTCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGCCCTCCAGGATAAAGGTTTCACTCGCCCGACCGCCATTCAGGCTGC > NZ_CP009273/2706193‑2706352 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |