Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,294,319	T→C	D106G (GAT→GGT)	ubiT ←	SCP2 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,294,319	0	T	C	100.0%	32.6 / NA	12	D106G (GAT→GGT)	ubiT	SCP2 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/4);  total (8/4)

GTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAAC  >  NZ_CP009273/3294237‑3294398                                                                                   |                                                                                gTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGACCTTGttt                                                                          >  2:446670/1‑90 (MQ=255)           tttttCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGACCTTGTTTACGCGCCGCa                                                                >  2:375388/1‑90 (MQ=255)            ttttCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGACCTTGTTTACGCGCCGCaa                                                               >  2:113598/1‑90 (MQ=255)                   aTACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGACCTTGTTTACGCGCCGCAATCATcag                                                        >  1:167351/1‑90 (MQ=255)                        gCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGACCTTGTTTACGCGCCGCAATCATCAGCAGAt                                                   >  2:335476/1‑90 (MQ=255)                                   gTATCGCCTTCAATCACCAGCCGACGCTGGCAGAAGAGCGTATCCGGACCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCg                                        <  1:452426/90‑1 (MQ=255)                                   gTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGACCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCg                                        <  1:346043/90‑1 (MQ=255)                                        gCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGACCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCGGCACt                                   >  1:80501/1‑90 (MQ=255)                                             cAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGACCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAAc                              <  1:151801/90‑1 (MQ=255)                                                    cAGCCGACGCTGGAAGAAGAGCGTATCCGGACCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATc                       <  1:335476/90‑1 (MQ=255)                                                                      gagCGTATCCGGACCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCt     >  2:292983/1‑90 (MQ=255)                                                                         cGTATCCGGACCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAAc  >  1:246815/1‑90 (MQ=255)                                                                                   |                                                                                GTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAAC  >  NZ_CP009273/3294237‑3294398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑