| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,305,625 | T→C | E171G (GAA→GGA) | truB ← | tRNA pseudouridine(55) synthase TruB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,305,625 | 0 | T | C | 100.0% | 39.1 / NA | 14 | E171G (GAA→GGA) | truB | tRNA pseudouridine(55) synthase TruB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/5); total (9/5) | |||||||||||
TAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAAT > NZ_CP009273/3305545‑3305707 | tAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTcccagctcca < 2:180347/90‑1 (MQ=255) aTGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTCCcagctccagctc > 2:170865/1‑90 (MQ=255) tGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTCCCAGCTCCAGCTCa > 2:89804/1‑90 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTCCCAGCTCCAGCTCAtt < 1:427544/90‑1 (MQ=255) gcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTCCCAGCTCCAGCTCATTg > 1:87748/1‑90 (MQ=255) gCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTCCCAGCTCCAGCTCATTGCCTTCATg < 2:356471/90‑1 (MQ=255) tgGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTCCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTaa > 2:171697/1‑90 (MQ=255) gTGCGGATATAAGTGCCTTTTGAGCAGTGAATTCCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATc > 1:279577/1‑90 (MQ=255) atatAAGTGCCTTTTGAGCAGTGAATTCCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGAcgc < 2:239686/90‑1 (MQ=255) aaGTGCCTTTTGAGCAGTGAATTCCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGAc > 1:374250/1‑84 (MQ=255) aaGTGCCTTTTGAGCAGTGAATTCCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGAc < 2:374250/84‑1 (MQ=255) gCCTTTTGAGCAGTGAATTCCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCAcg > 1:328297/1‑90 (MQ=255) tttGAGCAGTGAATTCCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGa > 1:344763/1‑90 (MQ=255) aGTGAATTCCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCaa < 2:254773/90‑1 (MQ=255) gTGAATTCCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAAt > 1:224436/1‑90 (MQ=255) | TAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAAT > NZ_CP009273/3305545‑3305707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |