Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,505,616	T→C	K30E (AAA→GAA)	BW25113_RS17530 ←	GntR family transcriptional regulator YhfZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,505,616	0	T	C	100.0%	27.6 / NA	11	K30E (AAA→GAA)	BW25113_RS17530	GntR family transcriptional regulator YhfZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/4);  total (7/4)

CTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAA  >  NZ_CP009273/3505532‑3505690                                                                                     |                                                                           cTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTCCAATc                                                                       >  2:247867/1‑90 (MQ=255)     gCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTCCAATCGAtt                                                                   <  2:365504/90‑1 (MQ=255)     gCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTCCAATCGAtt                                                                   >  2:62200/1‑90 (MQ=255)          aTTCCAGCGTTTGCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGTATTCATTTGCCAGATCATCTACTGTTTCCAATCGATTTCCGc                                                              <  2:180490/90‑1 (MQ=255)                                            aaCGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTCCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGcgtcgt                            >  1:169264/1‑90 (MQ=255)                                                      cGGCATTCATTTGCCAGCTCATCTATGGTTTCCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGacgacg                  <  1:233621/90‑1 (MQ=255)                                                          attcattTGCCAGCTCATCTATGGTTTCCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCctt              >  2:303813/1‑90 (MQ=255)                                                               tttGCCAGCTCATCTATGGTTTCCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCtttt         <  2:372819/90‑1 (MQ=255)                                                                      gCTCATCTATGGTTTCCAATCGATTACCGCACTTTTCACCTAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATaa  >  2:413124/1‑90 (MQ=255)                                                                      gCTCATCTATGGTTTCCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATaa  >  1:103084/1‑90 (MQ=255)                                                                      gCTCATCTATGGTTTCCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATaa  >  1:15663/1‑90 (MQ=255)                                                                                     |                                                                           CTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAA  >  NZ_CP009273/3505532‑3505690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑