| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,715,428 | T→G | intergenic (+19/+260) | BW25113_RS25300 → / ← glyS | IS3‑like element IS150 family transposase/glycine‑‑tRNA ligase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,715,428 | 0 | T | G | 85.0% | 50.7 / 2.7 | 20 | intergenic (+19/+260) | BW25113_RS25300/glyS | IS3‑like element IS150 family transposase/glycine‑‑tRNA ligase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base G (9/8); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
ATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACA > NZ_CP009273/3715341‑3715501 | aTTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGGCa < 2:32011/90‑1 (MQ=255)aTTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGGCa > 2:120761/1‑90 (MQ=255) aaaTTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAAC‑TTTTGGGGGCAGTACAAACtt < 2:252229/90‑1 (MQ=255) aaaGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGGCAGTACAAACTTTGAtt < 2:58165/90‑1 (MQ=255) aaaGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGGCAGTACAAACTTTGAtt < 2:282520/90‑1 (MQ=255) aaTTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGGCAGTACAAACTTTGAtt > 2:396417/1‑76 (MQ=255) aaTTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGGCAGTACAAACTTTGAtt < 1:396417/76‑1 (MQ=255) gACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGGCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCt > 1:164448/1‑90 (MQ=255) tataTGCCTCGTGTTTAACTGTCCAACTTTTTGGGGGCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCg > 2:130312/1‑90 (MQ=255) cTCGTGTTTAACTGTCCAACTTTTTGGGGGCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATa < 1:209693/90‑1 (MQ=255) tCGTGTTTAACTGTCCAACTTTTTGGGGGCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATAc > 1:196847/1‑90 (MQ=255) gtgtTTAACTGTCCAACTTTTTGGGGGCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCt > 1:284850/1‑90 (MQ=255) tgtTTAACTGTCCAACTTTTTGGGGGCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTg > 2:223286/1‑90 (MQ=255) tGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAAc < 1:429320/90‑1 (MQ=255) tGTCCAACTTTTTGGGGGCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAAc < 2:196847/90‑1 (MQ=255) tGTCCAACTTTTTGGGGGCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAAc < 2:36990/90‑1 (MQ=255) gTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACa > 1:390329/1‑90 (MQ=255) gTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACa > 1:25125/1‑90 (MQ=255) gTCCAACTTTTTGGGGGCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACa > 1:91689/1‑90 (MQ=255) gTCCAACTTTTTGGGGGCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACa > 2:270578/1‑90 (MQ=255) | ATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACA > NZ_CP009273/3715341‑3715501 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |